Optimization of Ultrasonic Extraction of Phenolic Compounds from Epimedium brevicornum Maxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In Vitro
The ultrasound-assisted extraction of phenolic compounds from Epimedium brevicornu Maxim was modeled using response surface methodology. A Central Composite Design (CCD) was employed to optimize three extraction variables, including ethanol concentration (X1), extraction time (X2), and ratio of aque...
| Published in: | Journal of Analytical Methods in Chemistry |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
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| Online Access: | http://dx.doi.org/10.1155/2014/864654 |
| _version_ | 1849437777924456448 |
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| author | Yan Zhao Yingying Hou Guosheng Tang Enbo Cai Shuangli Liu He Yang Lianxue Zhang Shijie Wang |
| author_facet | Yan Zhao Yingying Hou Guosheng Tang Enbo Cai Shuangli Liu He Yang Lianxue Zhang Shijie Wang |
| author_sort | Yan Zhao |
| collection | DOAJ |
| container_title | Journal of Analytical Methods in Chemistry |
| description | The ultrasound-assisted extraction of phenolic compounds from Epimedium brevicornu Maxim was modeled using response surface methodology. A Central Composite Design (CCD) was employed to optimize three extraction variables, including ethanol concentration (X1), extraction time (X2), and ratio of aqueous ethanol to raw material (X3), for the achievement of high extraction yield of the phenolic compounds. The optimized conditions are X1 of 50% (v/v), X2 of 27.5 min, and X3 of 250 mL/g. Under these conditions, the experimental yield is 4.29 ± 0.033% (n=3). The antioxidant activity was evaluated using the DPPH assay and ferric-reducing antioxidant power (FRAP). And it indicates that the phenolic compounds from Epimedium brevicornu Maxim possess significant antioxidant activity. HPLC analysis reveals that the main phenolic compound in the extract product was identified as gallic acid, catechin (Cianidanol), p-hydroxybenzoic acid, vanillic acid, caffeic acid, ferulaic acid, rutin, benzoic acid, and quercetin. |
| format | Article |
| id | doaj-art-5fb23401e1bb4d6183afcc42e5cb3b9d |
| institution | Directory of Open Access Journals |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| spelling | doaj-art-5fb23401e1bb4d6183afcc42e5cb3b9d2025-08-20T03:34:56ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732014-01-01201410.1155/2014/864654864654Optimization of Ultrasonic Extraction of Phenolic Compounds from Epimedium brevicornum Maxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In VitroYan Zhao0Yingying Hou1Guosheng Tang2Enbo Cai3Shuangli Liu4He Yang5Lianxue Zhang6Shijie Wang7College of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, ChinaCollege of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, ChinaCollege of Traditional Chinese Medicine, Jilin Agricultural Science and Technology University, Jilin 132101, ChinaThe ultrasound-assisted extraction of phenolic compounds from Epimedium brevicornu Maxim was modeled using response surface methodology. A Central Composite Design (CCD) was employed to optimize three extraction variables, including ethanol concentration (X1), extraction time (X2), and ratio of aqueous ethanol to raw material (X3), for the achievement of high extraction yield of the phenolic compounds. The optimized conditions are X1 of 50% (v/v), X2 of 27.5 min, and X3 of 250 mL/g. Under these conditions, the experimental yield is 4.29 ± 0.033% (n=3). The antioxidant activity was evaluated using the DPPH assay and ferric-reducing antioxidant power (FRAP). And it indicates that the phenolic compounds from Epimedium brevicornu Maxim possess significant antioxidant activity. HPLC analysis reveals that the main phenolic compound in the extract product was identified as gallic acid, catechin (Cianidanol), p-hydroxybenzoic acid, vanillic acid, caffeic acid, ferulaic acid, rutin, benzoic acid, and quercetin.http://dx.doi.org/10.1155/2014/864654 |
| spellingShingle | Yan Zhao Yingying Hou Guosheng Tang Enbo Cai Shuangli Liu He Yang Lianxue Zhang Shijie Wang Optimization of Ultrasonic Extraction of Phenolic Compounds from Epimedium brevicornum Maxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In Vitro |
| title | Optimization of Ultrasonic Extraction of Phenolic Compounds from Epimedium brevicornum Maxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In Vitro |
| title_full | Optimization of Ultrasonic Extraction of Phenolic Compounds from Epimedium brevicornum Maxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In Vitro |
| title_fullStr | Optimization of Ultrasonic Extraction of Phenolic Compounds from Epimedium brevicornum Maxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In Vitro |
| title_full_unstemmed | Optimization of Ultrasonic Extraction of Phenolic Compounds from Epimedium brevicornum Maxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In Vitro |
| title_short | Optimization of Ultrasonic Extraction of Phenolic Compounds from Epimedium brevicornum Maxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In Vitro |
| title_sort | optimization of ultrasonic extraction of phenolic compounds from epimedium brevicornum maxim using response surface methodology and evaluation of its antioxidant activities in vitro |
| url | http://dx.doi.org/10.1155/2014/864654 |
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