Protocol for the expansion of mouse immune effector cells for in vitro and in vivo studies

Summary: Reproducible and efficient expansion of different immune effector cells is required for pre-clinical studies investigating adoptive cell therapies against cancer. Here, we provide a protocol for the rapid expansion of mouse T cells, natural killer (NK) cells, and bone-marrow-derived macroph...

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Bibliographic Details
Published in:STAR Protocols
Main Authors: Thomas Look, Hanna Meister, Michael Weller, Tobias Weiss
Format: Article
Language:English
Published: Elsevier 2023-12-01
Subjects:
Cell Biology
Cell culture
Cell isolation
Flow Cytometry
Cancer
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723006676
Description
Summary:Summary: Reproducible and efficient expansion of different immune effector cells is required for pre-clinical studies investigating adoptive cell therapies against cancer. Here, we provide a protocol for the rapid expansion of mouse T cells, natural killer (NK) cells, and bone-marrow-derived macrophages (BMDMs). We describe steps for αCD3/αCD8 plate coating, isolating splenocytes, and expanding T cells and NK cells. Further, we detail procedures for bone marrow isolation and BMDM differentiation. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

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