Application of Doehlert Experimental Design for Optimization of a New-Based Hydrophilic Interaction Solid-Phase Extraction of Phenolic Acids from Olive Oils
In this work, a rapid, precise, and cost-valuable method has been established to quantify phenolic compounds in olive oil using new-based hydrophilic interaction solid-phase extraction (SPE). Boehlert’s experimental design applied the determination of the optimal operating conditions. An investigati...
| Published in: | Molecules |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/1420-3049/28/3/1073 |
| _version_ | 1850096511755485184 |
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| author | Bochra Bejaoui Kefi Khaoula Nefzi Sidrine Koumba Naceur M’Hamdi Patrick Martin |
| author_facet | Bochra Bejaoui Kefi Khaoula Nefzi Sidrine Koumba Naceur M’Hamdi Patrick Martin |
| author_sort | Bochra Bejaoui Kefi |
| collection | DOAJ |
| container_title | Molecules |
| description | In this work, a rapid, precise, and cost-valuable method has been established to quantify phenolic compounds in olive oil using new-based hydrophilic interaction solid-phase extraction (SPE). Boehlert’s experimental design applied the determination of the optimal operating conditions. An investigation into the effects of the methanol composition (50–100%), the volume of eluent (1–12 mL), and pH (1–3) on the extraction of phenols acids and total phenols from Tunisian olive oils was performed. The results showed that the extraction conditions had a significant effect on the extraction efficiency. The experiment showed that the greatest conditions for the SPE of phenolic acids were the methanol composition at 90.3%, pH at 2.9, and volume at 7.5 mL, respectively. The optimal conditions were applied to different types of olive oils, and it could be concluded that larger concentrations of polyphenols were found in extra virgin olive oil (89.15–218), whereas the lowest levels of these compounds (66.8 and 5.1) were found in cold-pressed crude olive oil and olive pomace oil, respectively. |
| format | Article |
| id | doaj-art-614c00a4d2f14bd990c96d806f769d2b |
| institution | Directory of Open Access Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-614c00a4d2f14bd990c96d806f769d2b2025-08-20T00:06:40ZengMDPI AGMolecules1420-30492023-01-01283107310.3390/molecules28031073Application of Doehlert Experimental Design for Optimization of a New-Based Hydrophilic Interaction Solid-Phase Extraction of Phenolic Acids from Olive OilsBochra Bejaoui Kefi0Khaoula Nefzi1Sidrine Koumba2Naceur M’Hamdi3Patrick Martin4Laboratory of Useful Materials, National Institute of Research and Pysico-Chemical Analysis (INRAP), Technopark of Sidi Thabet, Ariana 2020, TunisiaLR11INRGREF0 Laboratory of Management and Valorization of Forest Resources, National Research Institute of Rural Engineering, Water and Forests (INRGREF), Carthage University, Ariana 2080, TunisiaUnité Transformations & Agroressources, ULR7519, Université d’Artois-Uni LaSalle, F-62408 Bethune, FranceResearch Laboratory of Ecosystems & Aquatic Resources, National Agronomic Institute of Tunisia, Carthage University, 43 Avenue Charles Nicolle, Tunis 1082, TunisiaUnité Transformations & Agroressources, ULR7519, Université d’Artois-Uni LaSalle, F-62408 Bethune, FranceIn this work, a rapid, precise, and cost-valuable method has been established to quantify phenolic compounds in olive oil using new-based hydrophilic interaction solid-phase extraction (SPE). Boehlert’s experimental design applied the determination of the optimal operating conditions. An investigation into the effects of the methanol composition (50–100%), the volume of eluent (1–12 mL), and pH (1–3) on the extraction of phenols acids and total phenols from Tunisian olive oils was performed. The results showed that the extraction conditions had a significant effect on the extraction efficiency. The experiment showed that the greatest conditions for the SPE of phenolic acids were the methanol composition at 90.3%, pH at 2.9, and volume at 7.5 mL, respectively. The optimal conditions were applied to different types of olive oils, and it could be concluded that larger concentrations of polyphenols were found in extra virgin olive oil (89.15–218), whereas the lowest levels of these compounds (66.8 and 5.1) were found in cold-pressed crude olive oil and olive pomace oil, respectively.https://www.mdpi.com/1420-3049/28/3/1073Doehlert experimental designolive oilphenolic compoundssolid-phase extraction |
| spellingShingle | Bochra Bejaoui Kefi Khaoula Nefzi Sidrine Koumba Naceur M’Hamdi Patrick Martin Application of Doehlert Experimental Design for Optimization of a New-Based Hydrophilic Interaction Solid-Phase Extraction of Phenolic Acids from Olive Oils Doehlert experimental design olive oil phenolic compounds solid-phase extraction |
| title | Application of Doehlert Experimental Design for Optimization of a New-Based Hydrophilic Interaction Solid-Phase Extraction of Phenolic Acids from Olive Oils |
| title_full | Application of Doehlert Experimental Design for Optimization of a New-Based Hydrophilic Interaction Solid-Phase Extraction of Phenolic Acids from Olive Oils |
| title_fullStr | Application of Doehlert Experimental Design for Optimization of a New-Based Hydrophilic Interaction Solid-Phase Extraction of Phenolic Acids from Olive Oils |
| title_full_unstemmed | Application of Doehlert Experimental Design for Optimization of a New-Based Hydrophilic Interaction Solid-Phase Extraction of Phenolic Acids from Olive Oils |
| title_short | Application of Doehlert Experimental Design for Optimization of a New-Based Hydrophilic Interaction Solid-Phase Extraction of Phenolic Acids from Olive Oils |
| title_sort | application of doehlert experimental design for optimization of a new based hydrophilic interaction solid phase extraction of phenolic acids from olive oils |
| topic | Doehlert experimental design olive oil phenolic compounds solid-phase extraction |
| url | https://www.mdpi.com/1420-3049/28/3/1073 |
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