<i>Hyptis obtusiflora</i> C. Presl ex Benth Methanolic Extract Exhibits Anti-Inflammatory and Anti-Gastritis Activities via Suppressing AKT/NF-κB Pathway

• الحاوية / القاعدة: Plants
• المؤلفون الرئيسيون: Jieun Oh, Jae Youl Cho, Daewon Kim
• التنسيق: مقال
• اللغة: الإنجليزية
• منشور في: MDPI AG 2023-03-01
• الموضوعات: <i>Hyptis obtusiflora</i> C. Presl ex Benth; anti-inflammation; TLR4; AKT
• الوصول للمادة أونلاين: https://www.mdpi.com/2223-7747/12/5/1146

Inflammation is an indispensable part of the human body’s self-defense mechanism against external stimuli. The interactions between Toll-like receptors and microbial components trigger the innate immune system via NF-κB signaling, which regulates the overall cell signaling including inflammatory responses and immune modulations. The anti-inflammatory effects of <i>Hyptis obtusiflora</i> C. Presl ex Benth, which has been used as a home remedy for gastrointestinal disorders and skin disease in rural areas of Latin America, have not yet been studied. Here, we investigate the medicinal properties of <i>Hyptis obtusiflora</i> C. Presl ex Benth methanol extract (Ho-ME) for inflammatory response suppression. Nitric oxide secretion in RAW264.7 cells triggered by TLR2, 3, or 4 agonists was reduced by Ho-ME. Reduction of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and interleukin (IL)-1b mRNA expression was observed. Decreased transcriptional activity in TRIF- and MyD88-overexpressing HEK293T cells was detected with a luciferase assay. Additionally, serially downregulated phosphorylation of kinase in the NF-κB pathway by Ho-ME was discovered in lipopolysaccharide-treated RAW264.7 cells. Together with the overexpression of its constructs, AKT was identified as a target protein of Ho-ME, and its binding domains were reaffirmed. Moreover, Ho-ME exerted gastroprotective effects in an acute gastritis mouse model generated by the administration of HCl and EtOH. In conclusion, Ho-ME downregulates inflammation via AKT targeting in the NF-κB pathway, and the combined results support <i>Hyptis obtusiflora</i> as a new candidate anti-inflammatory drug.