| 总结: | ABSTRACT During poultry meat processing, multiple biocides can be applied to reduce foodborne pathogen contamination. Previous experiments have investigated the efficacy of several biocides, but few have investigated the efficacy of multiple biocides applied in series. Here, we evaluated the synergistic effects of peracetic acid (PAA) in combination with other commonly applied biocides approved for use in U.S. poultry meat processing: cetylpyridinium chloride (CPC), calcium hypochlorite (CH), and sodium hypochlorite (SH). Serotype-specific mixtures of Salmonella isolates (serotypes Typhimurium, Enteritidis, Kentucky, and Infantis) were exposed to PAA and other biocides, individually and in combination, in 96-well plates using broth culture. Mixtures were exposed to the first biocide for 15 s, the second biocide for 15 s, and chemical activity was then neutralized with Dey-Engley broth, and cultures were incubated overnight to determine recoverability via pH indicator. Across three trials, results were highly variable for all serotypes and combinations tested; however, responses to non-PAA biocides were uniform when applied individually. When applied in combination, order of application significantly impacted PAA tolerance (pd < 0.05). Application of any non-zero concentration of CH or SH also resulted in significantly lower PAA tolerance across all serotypes (pd < 0.05). Dilution could not be ruled out as the cause of the tolerance or the variability. Variable results highlight the need to test biocides on meat products to accurately evaluate efficacy for poultry meat processing.IMPORTANCESalmonella enterica is one of the most common causes of human foodborne illness attributed to consumption of contaminated poultry in the U.S. Because many different biocide antimicrobials are used in serial during poultry processing, we investigated the tolerance of four serotypes of non-typhoidal Salmonella to four biocides applied in serial. Although responses were variable, we concluded that any non-zero concentration of calcium or sodium hypochlorite applied before peracetic acid will decrease the PAA tolerance of all four serotypes. These data are primarily useful in planning applied studies of biocides on meat products for disinfection.
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