Practical Guide to In Vitro Clonal Propagation of Nicotiana benthamiana Using Axillary Shoot Induction
This protocol outlines a reliable method for the micropropagation of Nicotiana benthamiana using axillary shoot branching. Axillary shoot induction involves stimulating the outgrowth of dormant buds located at the leaf axils, allowing for the development of genetically stable shoots without callus f...
| الحاوية / القاعدة: | Bio-Protocol |
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| المؤلف الرئيسي: | |
| التنسيق: | مقال |
| اللغة: | الإنجليزية |
| منشور في: |
Bio-protocol LLC
2025-09-01
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| الوصول للمادة أونلاين: | https://bio-protocol.org/en/bpdetail?id=5443&type=0 |
| الملخص: | This protocol outlines a reliable method for the micropropagation of Nicotiana benthamiana using axillary shoot branching. Axillary shoot induction involves stimulating the outgrowth of dormant buds located at the leaf axils, allowing for the development of genetically stable shoots without callus formation or the use of exogenous plant growth regulators. Nodal explants are cultured on MS medium supplemented with kinetin and indole-3-butyric acid (IBA) to induce shoot formation. Isolated shoots are then transferred to hormone-free MS medium for rooting. This method is simple, reproducible, and supports rapid plant multiplication for downstream applications such as agroinfiltration or transient protein expression. |
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| تدمد: | 2331-8325 |