Implementation of Noninvasive Preimplantation Genetic Testing for Aneuploidy with Spent Blastocyst Medium into Clinical and Laboratory Practice: The First Successful Experience in Taiwan

• Published in: Fertility & Reproduction
• Main Authors: Chun-Kai Chen, Wei-Han Huang, Tiencheng Arthur Chang, Kuang-Han Chao, Wang-Fung Chiang, Hsin-Yi Yeh, Szu-Yu Shen
• Format: Article
• Language: English
• Published: World Scientific Publishing 2023-03-01
• Subjects: Noninvasive Preimplantation Genetic Testing for Aneuploidy (niPGT-A); Preimplantation Genetic Testing for Aneuploidy (PGT-A); Spent Blastocyst Medium (SBM)
• Online Access: https://www.worldscientific.com/doi/10.1142/S2661318223720015
• ISSN: 2661-3182; 2661-3174

Noninvasive preimplantation genetic testing for aneuploidy (niPGT-A) using cell-free DNA (cfDNA) present in the spent blastocyst medium (SBM) of human blastocysts has emerged as an alternative for classical preimplantation genetic testing for aneuploidy (PGT-A). The major benefit is to avoid possible damage to the trophectoderm and subsequently increase the implantation potential of the embryo. niPGT-A provides more information including the ploidy state of both trophectoderm and inner cell mass compared to invasive PGT-A in which only a few trophectoderm cells are assessed. This study was to report our first successful experience solely using niPGT-A to select embryos to transfer resulting in a successful delivery. This practical application is believed to be the first case report in Taiwan and can serve as an innovative model for other clinics to apply niPGT-A.