Silent hemoglobin variant during capillary electrophoresis: A case report

Abstract Hemoglobin (Hb) North Manchester [β51(D2) Pro→His; HBB:c.155 C>A] is a rare Hb β‐globin gene variant that affects glycated Hb measurement values, such as ion‐exchange high‐performance liquid chromatography, in patients with diabetes. This variant was first detected in the UK in 1998. Her...

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Bibliographic Details
Published in:Journal of Diabetes Investigation
Main Authors: Yanping Yuan, Xianghai Zhou, Leili Gao, Qian Ren, Linong Ji
Format: Article
Language:English
Published: Wiley 2020-07-01
Subjects:
Capillary electrophoresis
Glycated hemoglobin
Hemoglobin variant
Online Access:https://doi.org/10.1111/jdi.13222
Description
Summary:Abstract Hemoglobin (Hb) North Manchester [β51(D2) Pro→His; HBB:c.155 C>A] is a rare Hb β‐globin gene variant that affects glycated Hb measurement values, such as ion‐exchange high‐performance liquid chromatography, in patients with diabetes. This variant was first detected in the UK in 1998. Here, we describe the first case involving Hb North Manchester detected incidentally in a patient with type 2 diabetes in Northern China. The Hb variant was discovered by ion‐exchange high‐performance liquid chromatography, yet capillary electrophoresis of both glycated Hb program and Hb program failed to detect it. Subsequently, Sanger sequencing was carried out to help identify the Hb variant.
ISSN:2040-1116
2040-1124

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