| Summary: | (1) Background: Currently, no data are available on the vitamin B<sub>12</sub> content of an organic fertilizer product, viz. fermented poultry manure, or whether the organic fertilizer product contains vitamin B<sub>12</sub> or inactive corrinoids (or both). (2) Methods: This study conducted a microbiological assay to determine the vitamin B<sub>12</sub> content of various commercially available fermented poultry manure fertilizer products. (3) Results: The results varied from 1.4 μg to approximately 20 μg per 100 g of dry weight. In the bioautography analysis, selected products had two positive spots with identical <i>R</i><sub>f</sub> values of vitamin B<sub>12</sub> and pseudovitamin B<sub>12.</sub> High-performance liquid chromatography and liquid chromatography/electrospray ionization−mass spectrometry analyses of the selected products indicated that these fertilizers primarily contained vitamin B<sub>12</sub>. They also contained minor inactive cobamides such as pseudovitamin B<sub>12</sub>, 2-methyladenyl cobamide, and 2-methylmercaptoadenyl cobamide. (4) Conclusions: These results suggested that edible plants would enrich vitamin B<sub>12</sub> using fermented poultry manure organic fertilizer products.
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