Chemistry-Assisted Proteomic Profiling of O-GlcNAcylation

The modification on proteins with O-linked N-acetyl-β-D-glucosamine (O-GlcNAcylation) is essential for normal cell physiology. Dysregulation of O-GlcNAcylation leads to many human diseases, such as cancer, diabetes and neurodegenerative diseases. Recently, the functional role of O-GlcNAcylation in d...

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Bibliographic Details
Published in:Frontiers in Chemistry
Main Authors: Qiang Zhu, Wen Yi
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-06-01
Subjects:
O-GlcNAcylation
enrichment strategies
mass spectrometry
quantitative proteomics
chemical tools
Online Access:https://www.frontiersin.org/articles/10.3389/fchem.2021.702260/full
Description
Summary:The modification on proteins with O-linked N-acetyl-β-D-glucosamine (O-GlcNAcylation) is essential for normal cell physiology. Dysregulation of O-GlcNAcylation leads to many human diseases, such as cancer, diabetes and neurodegenerative diseases. Recently, the functional role of O-GlcNAcylation in different physiological states has been elucidated due to the booming detection technologies. Chemical approaches for the enrichment of O-GlcNAcylated proteins combined with mass spectrometry-based proteomics enable the profiling of protein O-GlcNAcylation in a system-wide level. In this review, we summarize recent progresses on the enrichment and proteomic profiling of protein O-GlcNAcylation.
ISSN:2296-2646

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