Improved secretory expression and characterization of thermostable xylanase and β-xylosidase from Pseudothermotoga thermarum and their application in synergistic degradation of lignocellulose

Xylanase and β-xylosidase are the key enzymes for hemicellulose hydrolysis. To further improve hydrolysis efficacy, high temperature hydrolysis with thermostable hemicellulases showed promise. In this study, thermostable xylanase (Xyn) and β-xylosidase (XynB) genes from Pseudothermotoga thermarum we...

Full description

Bibliographic Details
Published in:	Frontiers in Bioengineering and Biotechnology
Main Authors:	Jinkang Chen, Hao Qin, Chaoqun You, Lingfeng Long
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-09-01
Subjects:	xylanase xylosidase secretory expression molecular docking saccharification
Online Access:	https://www.frontiersin.org/articles/10.3389/fbioe.2023.1270805/full

Similar Items

Enzymatic Properties of Recombinant Thermophilic Xylanase from Pseudothermotoga thermarum and Its Application in Beer Brewing
by: LI Qingfei, LIU Yue, YAN Xing, NIE Xinling, LI Xiangqian, TAN Zhongbiao, SHI Hao
Published: (2023-05-01)

Xylanases from thermophilic archaea: A hidden treasure
by: Almudena Saavedra-Bouza, et al.
Published: (2023-01-01)

Co-production of Xylooligosaccharides and Xylose From Poplar Sawdust by Recombinant Endo-1,4-β-Xylanase and β-Xylosidase Mixture Hydrolysis
by: Qi Li, et al.
Published: (2021-02-01)

Improving the Specific Activity and Thermostability of Psychrophilic Xylosidase AX543 by Comparative Mutagenesis
by: Kungang Pan, et al.
Published: (2022-08-01)

Simultaneous Improvement of Final Product-Tolerance and Thermostability of GH39 Xylosidase for Prebiotic Production by Directed Evolution
by: Zirui Zhang, et al.
Published: (2022-09-01)

Characterization of Cross-Linked Enzyme Aggregates of the Y509E Mutant of a Glycoside Hydrolase Family 52 β-xylosidase from <i>G. stearothermophilus</i>
by: Gabriela Romero, et al.
Published: (2021-01-01)

Discovery of a Novel β-xylosidase with Xylanase Activity and Its Application in the Production of Xylitol from Corncob Xylan
by: Meng Liang, et al.
Published: (2023-06-01)

Characterization of a novel thermostable and xylose-tolerant GH 39 β-xylosidase from Dictyoglomus thermophilum
by: Qi Li, et al.
Published: (2018-05-01)

Reassigning the role of a mesophilic xylan hydrolysing family GH43 β-xylosidase from Bacteroides ovatus, BoExXyl43A as exo-β-1,4-xylosidase
by: Parmeshwar Vitthal Gavande, et al.
Published: (2024-01-01)

Bioconversion of wild Ipomoea pes-caprae and Suaeda fruticosa biomass: a novel application of thermostable xylanase from Neobacillus sedimentimangrovi
by: Rozina Rashid, et al.
Published: (2025-05-01)

Agarase, Amylase and Xylanase from <i>Halomonas meridiana</i>: A Study on Optimization of Coproduction for Biomass Saccharification
by: Sneeha Veerakumar, et al.
Published: (2022-09-01)

Characterization of the Highly Efficient Acid-Stable Xylanase and β-Xylosidase System from the Fungus <i>Byssochlamys spectabilis</i> ATHUM 8891 (<i>Paecilomyces variotii</i> ATHUM 8891)
by: Anastasia P. Galanopoulou, et al.
Published: (2021-05-01)

Hydrolytic Potential of Talaromyces thermophilus β-Xylosidase and Its Use for Continuous Xylose Production
by: Ines Maalej-Achouri, et al.
Published: (2013-01-01)

Thermostable Xylanase Production by Geobacillus sp. Strain DUSELR13, and Its Application in Ethanol Production with Lignocellulosic Biomass
by: Mohit Bibra, et al.
Published: (2018-09-01)

Ultrahigh-Throughput Screening of High-β-Xylosidase-Producing <i>Penicillium piceum</i> and Investigation of the Novel β-Xylosidase Characteristics
by: Zhaokun Zhang, et al.
Published: (2022-03-01)

Deleting an xylosidase-encoding gene VdxyL3 increases growth and pathogenicity of Verticillium dahlia
by: Yongtai Li, et al.
Published: (2024-07-01)

Optimization of Xylanase Production through Response Surface Methodology by Fusarium sp. BVKT R2 Isolated from forest soil and its applications in saccharification
by: Ramanjaneyulu Golla, et al.
Published: (2016-09-01)

Highly thermostable xylanase production from a thermophilic Geobacillus sp. strain WSUCF1 utilizing lignocellulosic biomass
by: Aditya eBhalla, et al.
Published: (2015-06-01)

Degradation of beechwood xylan using food-grade bacteria-like particles displaying β-xylosidase from Limosilactobacillus fermentum
by: Robie Vasquez, et al.
Published: (2025-06-01)

Influence of some sugars on xylanase production by Aspergillus awamori in solid state fermentation
by: Lemos Judith Liliana Solórzano, et al.
Published: (2002-01-01)

Improvement in catalytic activity and thermostability of a GH10 xylanase and its synergistic degradation of biomass with cellulase
by: Shuai You, et al.
Published: (2019-12-01)

Use of xylosidase 3C from Segatella baroniae to discriminate xylan non-reducing terminus substitution characteristics
by: Franz J. St John, et al.
Published: (2024-06-01)

Production, Purification, and Characterization of Thermostable Alkaline Xylanase From Anoxybacillus kamchatkensis NASTPD13
by: Punam Yadav, et al.
Published: (2018-05-01)

XynDZ5: A New Thermostable GH10 Xylanase
by: Dimitra Zarafeta, et al.
Published: (2020-04-01)

β-Xylosidase and β-mannosidase in combination improved growth performance and altered microbial profiles in weanling pigs fed a corn-soybean meal-based diet
by: Shaoshuai Liu, et al.
Published: (2019-11-01)

Cloning of novel bacterial xylanases from lignocellulose-enriched compost metagenomic libraries
by: Simo Ellilä, et al.
Published: (2019-08-01)

Production of Xylooligosaccharides from <i>Jiuzao</i> by Autohydrolysis Coupled with Enzymatic Hydrolysis Using a Thermostable Xylanase
by: Liqin Qin, et al.
Published: (2022-09-01)

Application of Thermostable Wheat Arabino-xylanase Xyn11A for Quality Improvement of Wholewheat Bread
by: Yuxi ZHANG, et al.
Published: (2024-12-01)

Improving Thermostability of GH11 Xylanase XynASP by the Design of Loop Region
by: Tongbiao Li, et al.
Published: (2022-08-01)

Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis
by: Ning Zhang, et al.
Published: (2023-01-01)

New Intrinsically Thermostable Xylanase Improves Broilers’ Growth Performance, Organ Weights, and Affects Intestinal Viscosity and pH
by: Veerle Van Hoeck, et al.
Published: (2021-12-01)

Three Molecular Modification Strategies to Improve the Thermostability of Xylanase XynA from <i>Streptomyces rameus</i> L2001
by: Weijia Zhu, et al.
Published: (2023-02-01)

Heterologous expression and characterization of xylose-tolerant GH 43 family β-xylosidase/α-L-arabinofuranosidase from Limosilactobacillus fermentum and its application in xylan degradation
by: Robie Vasquez, et al.
Published: (2025-03-01)

Improvement of β‐Xylosidase and Endoxylanase Activities in Talaromyces amestolkiae by Genetic Manipulation of the Transcriptional Activator XlnR
by: Ana Pozo‐Rodríguez, et al.
Published: (2025-05-01)

Studies on the characteristics of xylanase
by: LI Wei-fen, et al.
Published: (2001-01-01)

RETRACTED ARTICLE: Isolation, expression, and in silico profiling of a thermostable xylanase from Geobacillus stearothermophilus strain NASA267: insights into structural features and agro-waste valorization
by: Safaa M. Ali, et al.
Published: (2025-03-01)

Enzymatic degradation of polysaccharides in Chinese vinegar residue to produce alcohol and xylose
by: Kunyi Liu, et al.
Published: (2021-06-01)

Structural Characterization of β-Xylosidase XynB2 from <i>Geobacillus stearothermophilus</i> CECT43: A Member of the Glycoside Hydrolase Family GH52
by: Jose Antonio Gavira, et al.
Published: (2023-12-01)

Characterization and Phylogenetic Analysis of a Novel GH43 β-Xylosidase From Neocallimastix californiae
by: Marcus Stabel, et al.
Published: (2021-07-01)

Characterization of Zalaria obscura Y1223 hydrolases: implications for lignocellulose conversion
by: Jeremy J. Boonzaier, et al.
Published: (2025-07-01)