Generation of a homozygous and a heterozygous SNCA gene knockout human-induced pluripotent stem cell line by CRISPR/Cas9 mediated allele-specific tuning of SNCA expression

Aggregation of alpha-synuclein (aSyn) is closely linked to Parkinson's disease, probably due to the loss of physiological functions and/or gain of toxic functions of aggregated aSyn. Significant efforts have been made elucidating the physiological structure and function of aSyn, however, with l...

Full description

Bibliographic Details
Published in:	Stem Cell Research
Main Authors:	Yanni Schneider, Soeren Turan, Aron Koller, Mandy Krumbiegel, Michaela Farrell, Sonja Plötz, Jürgen Winkler, Wei Xiang
Format:	Article
Language:	English
Published:	Elsevier 2022-12-01
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506122003014

Similar Items

DEVELOPMENT OF MURINE STEM CELLS WITH CONDITIONAL KNOCKOUT OF HUMANIZED SNCA GENE
by: E. A. Patrakhanov, et al.
Published: (2023-02-01)

Polymorphisms in the <i>SNCA</i> gene and the risk of synucleopathy
by: Natalia Yu. Abramycheva, et al.
Published: (2025-04-01)

Neuronal SNCA transcription during Lewy body formation
by: Tomoya Kon, et al.
Published: (2023-11-01)

Targeting SNCA in the treatment of malignant ascites in gastrointestinal cancer
by: Chie Kudo-Saito, et al.
Published: (2024-10-01)

Festination Correlates with SNCA Polymorphism in Chinese Patients with Parkinson’s Disease
by: Jinhua Zheng, et al.
Published: (2017-01-01)

Resequencing the complete SNCA locus in Indian patients with Parkinson’s disease
by: Asha Kishore, et al.
Published: (2024-04-01)

SNCA 3′ UTR Genetic Variants in Patients with Parkinson’s Disease
by: Antonela Blažeković, et al.
Published: (2021-11-01)

Generation of homozygous and heterozygous REEP1 knockout induced pluripotent stem cell lines by CRISPR/Cas9 gene editing
by: M. Korneck, et al.
Published: (2024-06-01)

Generation of an induced pluripotent stem cell line (CSC-32) from a patient with Parkinson's disease carrying a heterozygous variation p.A53T in the SNCA gene
by: Carla Azevedo, et al.
Published: (2020-03-01)

SNCA: Semi-Supervised Node Classification for Evolving Large Attributed Graphs
by: Faima Abbasi, et al.
Published: (2024-09-01)

Novel Therapeutic Horizons: <i>SNCA</i> Targeting in Parkinson’s Disease
by: Alessio Maria Caramiello, et al.
Published: (2024-08-01)

Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats
by: Yajie Wang, et al.
Published: (2023-05-01)

EVALUATING THE ROLE OF SNCA, GBA AND LRRK2 IN THE PATHOGENESIS OF PARKINSON’S DISEASE
by: Alicia Bautista-Garcia, et al.
Published: (2023-10-01)

The rs3857059 variant of the SNCA gene is associated with Parkinson’s disease in Mexican Mestizos
by: S. García, et al.
Published: (2016-06-01)

The rs3857059 variant of the SNCA gene is associated with Parkinson’s disease in Mexican Mestizos
by: S. García, et al.
Published: (2016-06-01)

Differential methylation of SNCA and MAPT genes associated with Parkinson’s disease in Mexican Mestizos
by: Ernesto G. Miranda-Morales, et al.
Published: (2025-07-01)

Genetic variants of the alpha-synuclein gene SNCA are associated with multiple system atrophy.
by: Ammar Al-Chalabi, et al.
Published: (2009-09-01)

A personalised and comprehensive approach is required to suppress or replenish SNCA for Parkinson’s disease
by: Dunhui Li, et al.
Published: (2025-03-01)

SNCA and TPPP transcripts increase in oligodendroglial cytoplasmic inclusions in multiple system atrophy
by: Tomoya Kon, et al.
Published: (2024-08-01)

Application of serum peptidomics for Parkinson's disease in SNCA-A30P mice
by: Zi Yin, et al.
Published: (2023-12-01)

Four Copies of SNCA Responsible for Autosomal Dominant Parkinson’s Disease in Two Italian Siblings
by: Rosangela Ferese, et al.
Published: (2015-01-01)

Decreased SNCA expression in whole-blood RNA analysis of Parkinson’s disease adjusting for neutrophils
by: Kayla Y. Xu, et al.
Published: (2025-10-01)

Meta-analysis and in-silico functional characterization of the SNCA variant rs356220 in Parkinson’s disease
by: Shradha Menon, et al.
Published: (2025-07-01)

Genetic Impact on Clinical Features in Parkinson’s Disease: A Study on SNCA-rs11931074
by: Li Shu, et al.
Published: (2018-01-01)

Neuronal activity triggers secretory autophagy to mediate the extracellular release of SNCA/α-synuclein
by: Yoshitsugu Nakamura, et al.
Published: (2024-12-01)

Targeted attenuation of elevated histone marks at SNCA alleviates α‐synuclein in Parkinson's disease
by: Subhrangshu Guhathakurta, et al.
Published: (2021-01-01)

Genetic Variants in SNCA and the Risk of Sporadic Parkinson’s Disease and Clinical Outcomes: A Review
by: Clarissa Loureiro das Chagas Campêlo, et al.
Published: (2017-01-01)

SNCA genetic lowering reveals differential cognitive function of alpha-synuclein dependent on sex
by: Jennifer L. Brown, et al.
Published: (2022-12-01)

Linking the phenotype of SNCA Triplication with PET-MRI imaging pattern and alpha-synuclein CSF seeding
by: Isabel Wurster, et al.
Published: (2022-09-01)

Regulatory roles of eugenol in paraquat-altered SNCA/LZTS3/MAPT in the cerebellum of Wistar rats
by: Obinna Onwe Uchewa, et al.
Published: (2025-01-01)

Neuronal-type-specific epigenome editing to decrease SNCA expression: Implications for precision medicine in synucleinopathies
by: Zhiguo Sun, et al.
Published: (2024-03-01)

Correction: SNCA genetic lowering reveals differential cognitive function of alpha-synuclein dependent on sex
by: Jennifer L. Brown, et al.
Published: (2024-06-01)

A systematic review of associations between common SNCA variants and clinical heterogeneity in Parkinson’s disease
by: Camilla Christina Pedersen, et al.
Published: (2021-07-01)

Establishment of heterozygous and homozygous SHANK3 knockout clonal pluripotent stem cells from the parental hESC line SA001 using CRISPR/Cas9
by: Laure Chatrousse, et al.
Published: (2023-10-01)

Alpha‐synuclein dynamics in induced pluripotent stem cell‐derived dopaminergic neurons from a Parkinson’s disease patient (PARK4) with SNCA triplication
by: Hayato Fukusumi, et al.
Published: (2021-02-01)

Generation of three clones (JUCGRMi002-A, B, C) of induced pluripotent stem cells from a Parkinson’s disease patient with SNCA duplication
by: Kei-ichi Ishikawa, et al.
Published: (2024-02-01)

RNA binding protein AUF1/HNRNPD regulates nuclear export, stability and translation of SNCA transcripts
by: Fedon-Giasin Kattan, et al.
Published: (2023-11-01)

Investigating the relationship between the SNCA gene and cognitive abilities in idiopathic Parkinson’s disease using machine learning
by: Mehrafarin Ramezani, et al.
Published: (2021-03-01)

Variants in Lrrk2 and Snca deficiency do not alter the course of primary encephalitis due to neurotropic reovirus T3D in newborn mice
by: Michaela O. Lunn, et al.
Published: (2025-01-01)

Identification of SNCA and DRD2 as key genes linking parkinson’s disease and circadian rhythm through bioinformatics analysis
by: Jie Zhang, et al.
Published: (2025-08-01)