In vitro evaluation of photon and carbon ion radiotherapy in combination with cisplatin in head and neck squamous cell carcinoma cell lines
BackgroundHeavy ion radiotherapy, such as carbon ion radiotherapy (CIRT), has multiple advantages over conventional photon therapy. Cisplatin, as a classic anti-tumor drugs, has been tested and discovered as a photon radiosensitizer in several cell lines, including head and neck squamous cell carcin...
| Published in: | Frontiers in Oncology |
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Frontiers Media S.A.
2023-04-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.896142/full |
| _version_ | 1852649597166419968 |
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| author | Xumeng Fang Xumeng Fang Xumeng Fang Pian Sun Pian Sun Pian Sun Yuanli Dong Yuanli Dong Yuanli Dong Yangle Huang Yangle Huang Yangle Huang Jiade Jay Lu Jiade Jay Lu Jiade Jay Lu Lin Kong Lin Kong Lin Kong |
| author_facet | Xumeng Fang Xumeng Fang Xumeng Fang Pian Sun Pian Sun Pian Sun Yuanli Dong Yuanli Dong Yuanli Dong Yangle Huang Yangle Huang Yangle Huang Jiade Jay Lu Jiade Jay Lu Jiade Jay Lu Lin Kong Lin Kong Lin Kong |
| author_sort | Xumeng Fang |
| collection | DOAJ |
| container_title | Frontiers in Oncology |
| description | BackgroundHeavy ion radiotherapy, such as carbon ion radiotherapy (CIRT), has multiple advantages over conventional photon therapy. Cisplatin, as a classic anti-tumor drugs, has been tested and discovered as a photon radiosensitizer in several cell lines, including head and neck squamous cell carcinoma (HNSCC). Hence, the aim of our study is to evaluate whether cisplatin can sensitize CIRT towards HNSCC cell lines in vitro.MethodsHuman nasopharyngeal carcinoma cell line CNE-2, human tongue squamous carcinoma cell line TCA 8113 and human hypopharynx squamous carcinoma cell line FADU were all irradiated with photon beam of 2, 4, 6, 8 Gy (physical dose) and carbon ion beam of 1, 2, 3, 4 Gy (physical dose) and treated with cisplatin. Cell survival was assessed by clonogenic survival assay.ResultsCIRT showed significantly stronger cytotoxic effect than standard photon radiotherapy. The relative biological effectiveness (RBE) of carbon ion beam at 10% survival (RBE10) was calculated 3.07 for CNE-2, 2.33 for TCA 8113 and 2.36 for FADU. Chemoradiotherapy (both photon radiotherapy and CIRT) was more effective than radiotherapy alone. In vitro sensitizer enhancement ratios (SERs) of cisplatin in CNE-2, TCA 8113 and FA DU cell lines after photon irradiation were 1.33, 1.14 and 1.21, while after carbon ion irradiation were 1.02, 1.00 and 0.96, showed that cisplatin sensitized photon irradiation but showed no sensitization effect in carbon ion irradiation in all tested cell lines.ConclusionsIn conclusion, high linear energy transfer (LET) CIRT was more effective than photon irradiation to prevent the proliferation of HNSCC cell lines. Additional treatment with cisplatin could sensitize photon irradiation but showed no effect on carbon ion irradiation. |
| format | Article |
| id | doaj-art-7cf3ddfcbeea47d9b445308a98b03ded |
| institution | Directory of Open Access Journals |
| issn | 2234-943X |
| language | English |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| spelling | doaj-art-7cf3ddfcbeea47d9b445308a98b03ded2025-08-19T21:41:40ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-04-011310.3389/fonc.2023.896142896142In vitro evaluation of photon and carbon ion radiotherapy in combination with cisplatin in head and neck squamous cell carcinoma cell linesXumeng Fang0Xumeng Fang1Xumeng Fang2Pian Sun3Pian Sun4Pian Sun5Yuanli Dong6Yuanli Dong7Yuanli Dong8Yangle Huang9Yangle Huang10Yangle Huang11Jiade Jay Lu12Jiade Jay Lu13Jiade Jay Lu14Lin Kong15Lin Kong16Lin Kong17Department of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Fudan University Cancer Hospital, Shanghai, ChinaShanghai Key Laboratory of Radiation Oncology (20dz2261000), Shanghai, ChinaShanghai Engineering Research Center of Proton and Heavy Ion Radiation Therapy, Shanghai, ChinaDepartment of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Fudan University Cancer Hospital, Shanghai, ChinaShanghai Key Laboratory of Radiation Oncology (20dz2261000), Shanghai, ChinaShanghai Engineering Research Center of Proton and Heavy Ion Radiation Therapy, Shanghai, ChinaDepartment of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Fudan University Cancer Hospital, Shanghai, ChinaShanghai Key Laboratory of Radiation Oncology (20dz2261000), Shanghai, ChinaShanghai Engineering Research Center of Proton and Heavy Ion Radiation Therapy, Shanghai, ChinaDepartment of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Fudan University Cancer Hospital, Shanghai, ChinaShanghai Key Laboratory of Radiation Oncology (20dz2261000), Shanghai, ChinaShanghai Engineering Research Center of Proton and Heavy Ion Radiation Therapy, Shanghai, ChinaDepartment of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Fudan University Cancer Hospital, Shanghai, ChinaShanghai Key Laboratory of Radiation Oncology (20dz2261000), Shanghai, ChinaShanghai Engineering Research Center of Proton and Heavy Ion Radiation Therapy, Shanghai, ChinaDepartment of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Fudan University Cancer Hospital, Shanghai, ChinaShanghai Key Laboratory of Radiation Oncology (20dz2261000), Shanghai, ChinaShanghai Engineering Research Center of Proton and Heavy Ion Radiation Therapy, Shanghai, ChinaBackgroundHeavy ion radiotherapy, such as carbon ion radiotherapy (CIRT), has multiple advantages over conventional photon therapy. Cisplatin, as a classic anti-tumor drugs, has been tested and discovered as a photon radiosensitizer in several cell lines, including head and neck squamous cell carcinoma (HNSCC). Hence, the aim of our study is to evaluate whether cisplatin can sensitize CIRT towards HNSCC cell lines in vitro.MethodsHuman nasopharyngeal carcinoma cell line CNE-2, human tongue squamous carcinoma cell line TCA 8113 and human hypopharynx squamous carcinoma cell line FADU were all irradiated with photon beam of 2, 4, 6, 8 Gy (physical dose) and carbon ion beam of 1, 2, 3, 4 Gy (physical dose) and treated with cisplatin. Cell survival was assessed by clonogenic survival assay.ResultsCIRT showed significantly stronger cytotoxic effect than standard photon radiotherapy. The relative biological effectiveness (RBE) of carbon ion beam at 10% survival (RBE10) was calculated 3.07 for CNE-2, 2.33 for TCA 8113 and 2.36 for FADU. Chemoradiotherapy (both photon radiotherapy and CIRT) was more effective than radiotherapy alone. In vitro sensitizer enhancement ratios (SERs) of cisplatin in CNE-2, TCA 8113 and FA DU cell lines after photon irradiation were 1.33, 1.14 and 1.21, while after carbon ion irradiation were 1.02, 1.00 and 0.96, showed that cisplatin sensitized photon irradiation but showed no sensitization effect in carbon ion irradiation in all tested cell lines.ConclusionsIn conclusion, high linear energy transfer (LET) CIRT was more effective than photon irradiation to prevent the proliferation of HNSCC cell lines. Additional treatment with cisplatin could sensitize photon irradiation but showed no effect on carbon ion irradiation.https://www.frontiersin.org/articles/10.3389/fonc.2023.896142/fullhead and neck squamous cell carcinoma (HNSCC)cisplatincarbon ion radiotherapy (CIRT)relative biological effectiveness (RBE)radiosensitisation |
| spellingShingle | Xumeng Fang Xumeng Fang Xumeng Fang Pian Sun Pian Sun Pian Sun Yuanli Dong Yuanli Dong Yuanli Dong Yangle Huang Yangle Huang Yangle Huang Jiade Jay Lu Jiade Jay Lu Jiade Jay Lu Lin Kong Lin Kong Lin Kong In vitro evaluation of photon and carbon ion radiotherapy in combination with cisplatin in head and neck squamous cell carcinoma cell lines head and neck squamous cell carcinoma (HNSCC) cisplatin carbon ion radiotherapy (CIRT) relative biological effectiveness (RBE) radiosensitisation |
| title | In vitro evaluation of photon and carbon ion radiotherapy in combination with cisplatin in head and neck squamous cell carcinoma cell lines |
| title_full | In vitro evaluation of photon and carbon ion radiotherapy in combination with cisplatin in head and neck squamous cell carcinoma cell lines |
| title_fullStr | In vitro evaluation of photon and carbon ion radiotherapy in combination with cisplatin in head and neck squamous cell carcinoma cell lines |
| title_full_unstemmed | In vitro evaluation of photon and carbon ion radiotherapy in combination with cisplatin in head and neck squamous cell carcinoma cell lines |
| title_short | In vitro evaluation of photon and carbon ion radiotherapy in combination with cisplatin in head and neck squamous cell carcinoma cell lines |
| title_sort | in vitro evaluation of photon and carbon ion radiotherapy in combination with cisplatin in head and neck squamous cell carcinoma cell lines |
| topic | head and neck squamous cell carcinoma (HNSCC) cisplatin carbon ion radiotherapy (CIRT) relative biological effectiveness (RBE) radiosensitisation |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.896142/full |
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