Transcriptomic analysis of tobacco flowering regulated by the BdFKF1 gene in Brachypodium distachyon

• Published in: Guangxi Zhiwu
• Main Author: YANG Longjiao1, LI Yajiao2, CHEN Xi2, MA Peijie2, LUO Wenju1, CHEN Caijun2, CHEN Ying2, LIU Xiaoxia2, WANG Xiaoli 2*
• Format: Article
• Language: Chinese
• Published: China Science Publishing & Media Ltd. (CSPM) 2025-08-01
• Subjects: fkf1 gene; photoperiodism; tobacco; brachypodium distachyon
• Online Access: http://www.guihaia-journal.com/gxzw/ch/reader/create_pdf.aspx?file_no=20250814&year_id=2025&quarter_id=8&falg=1
• ISSN: 1000-3142

FKF1 is known to be a blue light-responsive gene and an important factor in regulating plant flowering through the photoperiod pathway. To explore the molecular mechanism of BdFKF1 gene in regulating flowering in tobacco plants through the photoperiod pathway, wild-type tobacco(SR1)and BdFKF1 gene-transformed tobacco plants(BdFKF1-OE)were used as materials. Transcriptomic sequencing and RT-qPCR validation were conducted to observe and record the flowering time in both materials. The results were as follows:(1)In SR1 vs FKF1 group, a total of 472 differentially expressed genes were identified, with 248 up-regulated genes and 224 down-regulated genes. Among them, 14 differentially expressed genes were related to the photoperiod, with 7 up-regulated genes and 7 down-regulated genes.(2)GO enrichment analysis revealed that differentially expressed genes were significantly enriched in pathways such as U5 snRNP, oxidoreductase activity, and acting on paired donors and response to blue light. Among them, the differentially expressed genes related to the photoperiod were mainly enriched in pathways such as FK506 binding, photoperiodism and flowering, photoperiodism, phosphatidylethanolamine binding, macrolide binding, and regulation of flower development.(3)KEGG enrichment analysis revealed that the differentially expressed genes were mainly enriched in pathways such as ABC transporters, Protein processing in endoplasmic reticulum, cutin, suberin and wax biosynthesis and circadian rhythm-plant pathways. Among them, the differentially expressed genes related to the photoperiod were mainly enriched in pathways such as plant hormone signal transduction and circadian rhythm-plant.(4)The observation and recording showed that the flowering time of BdFKF1-OE plants was advanced by 3.9 d compared to SR1.(5)RT-qPCR results were consistent with the trend of changes in the transcriptomic data, indicating that the transcriptomic data had high reliability. In conclusion, under long-day conditions, the BdFKF1 gene can affect the expression of photoperiod pathway-related genes, and overexpression of the BdFKF1 gene promotes flowering in tobacco plants.