Identification and preclinical efficacy evaluation of two lytic bacteriophages targeting highly virulent and multidrug-resistant Klebsiella pneumoniae

Abstract Background The emergence of MDR K. pneumoniae poses a critical challenge in treating respiratory-associated pneumonia. Bacteriophages are promising antibiotic alternatives with unique features. This study aimed to isolate new bacteriophages from the hospital environment and investigate thei...

Full description

Bibliographic Details
Published in:	Annals of Clinical Microbiology and Antimicrobials
Main Authors:	Ai Guo, Dianbao Zuo, Li Shi, Ming Guo, Jinquan Li, Caili Li, Puqing Wang, Xiaodong Sun, Ming Sang
Format:	Article
Language:	English
Published:	BMC 2025-08-01
Subjects:	Lytic bacteriophage Multidrug-resistant Klebsiella pneumoniae Bacteriophage therapy Biofilm degradation Histopathology
Online Access:	https://doi.org/10.1186/s12941-025-00812-9

Similar Items

Characterization and Genomic Analysis of a Novel Drexlervirial Bacteriophage IME268 with Lytic Activity Against Klebsiella pneumoniae
by: Nazir A, et al.
Published: (2022-04-01)

Characterization of two lytic bacteriophages infecting carbapenem-resistant clinical Klebsiella pneumoniae in Dhaka, Bangladesh
by: Nishat Tasnim Ananna, et al.
Published: (2024-12-01)

Evaluation of lytic bacteriophages for control of multidrug-resistant Salmonella Typhimurium
by: Lae-seung Jung, et al.
Published: (2017-09-01)

Influencing factors on the preservation of lytic bacteriophage VP3
by: Yue Xiao, et al.
Published: (2022-10-01)

Lytic Activity Spectra of Bacteriophages
by: I. M. Pchelin, et al.
Published: (2024-03-01)

Isolation and Characterization of Highly Lytic Bacteriophages against Staphylococcus aureus in Malaysian Sewage Water
by: Jyng Sheng Tee, et al.
Published: (2024-12-01)

Isolation and characterization of lytic bacteriophage from waste water to control clinical multidrug resistant Klebsiella pneumoniae
by: Muhammad R.A. Shah, et al.
Published: (2023-10-01)

Characterization of an Indigenous Lytic Phage Targeting Multidrug-Resistant Salmonella enterica
by: Ebele Onuigbo, et al.
Published: (2025-12-01)

Phage Therapy Against Biofilm of Multidrug-Resistant Klebsiella Pneumoniae Isolated from Zakho Hospital Samples
by: Yousif Abdullah AlBany, et al.
Published: (2019-06-01)

Isolation and Characterization of Novel Lytic Bacteriophages Infecting Epidemic Carbapenem-Resistant Klebsiella pneumoniae Strains
by: Min Li, et al.
Published: (2020-07-01)

Presence and Persistence of Putative Lytic and Temperate Bacteriophages in Vaginal Metagenomes from South African Adolescents
by: Anna-Ursula Happel, et al.
Published: (2021-11-01)

Isolation, characterization, therapeutic and prophylactic applications of a lytic bacteriophage to combat multi-drug resistance Shigella flexneri: an animal study model
by: Abbasi Fashami, Parisa, et al.
Published: (2025-04-01)

Genomic characterization, in vitro, and preclinical evaluation of two microencapsulated lytic phages VB_ST_E15 and VB_ST_SPNIS2 against clinical multidrug-resistant Salmonella serovars
by: Reem A. Youssef, et al.
Published: (2024-02-01)

A New Approach for Controlling <i>Agrobacterium tumefaciens</i> Post Transformation Using Lytic Bacteriophage
by: Fiqih Ramadhan, et al.
Published: (2022-11-01)

Isolation and Characterization of a Klebsiella pneumoniae Specific Lytic Bacteriophage from a Hospital Waste-water Treatment Plant
by: Mahboubeh Soleimani Sasani, et al.
Published: (2019-01-01)

Molecular studies of phages-Klebsiella pneumoniae in mucoid environment: innovative use of mucolytic agents prior to the administration of lytic phages
by: Olga Pacios, et al.
Published: (2023-10-01)

Isolation and Characterization of Effective Lytic Bacteriophages to Control the Antibiotic Resistant Klebsiella Pneumoniae Infections in Noth-West of Iran
by: Raheleh Majdani, et al.
Published: (2024-04-01)

Selection criteria for bacterial strains and bacteriophages for the formation of industrial collection of specifically lysing bacteria: <i>Klebsiella, Echerichia, Proteus, Pseudomonas, Staphylococcus</i>
by: E. N. Siatchikhina, et al.
Published: (2018-02-01)

Isolation, characterization, and application of a novel polyvalent lytic phage STWB21 against typhoidal and nontyphoidal Salmonella spp.
by: Payel Mondal, et al.
Published: (2022-08-01)

Evaluating phage lytic activity: from plaque assays to single-cell technologies
by: Vladimir Panteleev, et al.
Published: (2025-08-01)

Isolation and characterization of two novel bacteriophages against carbapenem-resistant Klebsiella pneumoniae
by: Abrar Senhaji-Kacha, et al.
Published: (2024-08-01)

Designing phage cocktails to combat the emergence of bacteriophage-resistant mutants in multidrug-resistant Klebsiella pneumoniae
by: Seongjun Yoo, et al.
Published: (2024-01-01)

Mucin and Agitation Shape Predation of <i>Escherichia coli</i> by Lytic Coliphage
by: Amanda Carroll-Portillo, et al.
Published: (2023-02-01)

Characterization of antibacterial activity of lytic bacteriophage PE-1 for biological control of Escherichia coli K88 in vitro
by: Penghao Zhao, et al.
Published: (2023-06-01)

Utility of lytic bacteriophage in the treatment of multidrug-resistant <i> Pseudomonas aeruginosa</i> septicemia in mice
by: Vinodkumar C, et al.
Published: (2008-07-01)

Isolation and characterization of lytic bacteriophages from various sources in Addis Ababa against antimicrobial-resistant diarrheagenic Escherichia coli strains and evaluation of their therapeutic potential
by: Tamirat Salile Sada, et al.
Published: (2024-03-01)

Evaluation of the therapeutic effect of a novel bacteriophage in the healing process of infected wounds with Klebsiella pneumoniae in mice
by: Fatemeh Zeynali Kelishomi, et al.
Published: (2024-03-01)

Isolation and characterization of lytic bacteriophage vB_KpnP_23: A promising antimicrobial candidate against carbapenem-resistant Klebsiella pneumoniae
by: Qian Wang, et al.
Published: (2024-12-01)

Isolation, characterization and therapeutic efficacy of lytic bacteriophage ZK22 against Salmonella Typhimurium in mice
by: Yihong Sun, et al.
Published: (2025-01-01)

Complete genome sequence of lytic bacteriophage RG-2014 that infects the multidrug resistant bacterium Delftia tsuruhatensis ARB-1
by: Ananda Shankar Bhattacharjee, et al.
Published: (2017-12-01)

Isolation and characterization of a novel lytic phage K14-2 infecting diverse species of the genus Klebsiella and Raoultella
by: Seomin Kang, et al.
Published: (2025-01-01)

Lytics broadcasting system: A novel approach to disseminate bacteriophages for disinfection and biogenic hydrogen sulphide removal tested in synthetic sewage
by: Amrita Salim, et al.
Published: (2022-03-01)

The potential use of bacteriophages as antibacterial agents against Klebsiella pneumoniae
by: Omid Gholizadeh, et al.
Published: (2024-08-01)

Genomic analysis and characterization of lytic bacteriophages that target antimicrobial resistant Escherichia coli in Addis Ababa, Ethiopia
by: Tamirat Salile Sada, et al.
Published: (2024-11-01)

Analysis of a novel phage as a promising biological agent targeting multidrug resistant Klebsiella pneumoniae
by: Mahmoud A. Abdel-Razek, et al.
Published: (2025-03-01)

Co-Occurrence of Multidrug Resistant Klebsiella pneumoniae Pathogenic Clones of Human Relevance in an Equine Pneumonia Case
by: Carola Venturini, et al.
Published: (2022-06-01)

K. OXYTOCA BACTERIOPHAGES ISOLATION METHODS IMPROVEMENT
by: G. R. Sadrtdinova
Published: (2018-01-01)

Novel Klebsiella pneumoniae K23-Specific Bacteriophages From Different Families: Similarity of Depolymerases and Their Therapeutic Potential
by: Roman B. Gorodnichev, et al.
Published: (2021-08-01)

Bacteriophages, a Solution to the Destruction of Antibiotic-Resistant Bacteria
by: Daniela Moţ, et al.
Published: (2024-05-01)

Characterization of a Multidrug-resistant Escherichia Coli Lytic Bacteriophage Isolate as a Safe Alternative to Synthetic Antibiotics
by: Najwa Alharbi, et al.
Published: (2024-12-01)