CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen production

Cassava (Manihot esculenta) is a starchy root crop that supports over a billion people in tropical and subtropical regions of the world. This staple, however, produces the neurotoxin cyanide and requires processing for safe consumption. Excessive consumption of insufficiently processed cassava, in c...

Full description

Bibliographic Details
Published in:	Frontiers in Plant Science
Main Authors:	Michael A. Gomez, Kodiak C. Berkoff, Baljeet K. Gill, Anthony T. Iavarone, Samantha E. Lieberman, Jessica M. Ma, Alex Schultink, Nicholas G. Karavolias, Stacia K. Wyman, Raj Deepika Chauhan, Nigel J. Taylor, Brian J. Staskawicz, Myeong-Je Cho, Daniel S. Rokhsar, Jessica B. Lyons
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-03-01
Subjects:	cassava (Manihot esculenta Crantz) cyanide cyanogenesis CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 (CRISPR-associated protein 9) genome editing CYP79D
Online Access:	https://www.frontiersin.org/articles/10.3389/fpls.2022.1079254/full

Similar Items

Transposon signatures of allopolyploid genome evolution
by: Adam M. Session, et al.
Published: (2023-06-01)

Plant Cyanogenic-Derived Metabolites and Herbivore Counter-Defences
by: Manuel Martinez, et al.
Published: (2024-04-01)

Cyanogenic glycosides can function as nitrogen reservoir for flax plants cultured under N-deficient conditions
by: Irena Siegień, et al.
Published: (2021-04-01)

An Inducible CYP19A1 Excision Model for Sexual Differentiation in Chicken (<i>Gallus gallus</i>) via the CRISPR/Cas9 System
by: Guangzheng Liu, et al.
Published: (2025-03-01)

CRISPR genome editing and its medical applications
by: Mohammad-Reza Mahmoudian-sani, et al.
Published: (2018-03-01)

Approaches for timeline reductions in pathogenesis studies using genetically modified mice
by: Samantha Skavicus, et al.
Published: (2023-10-01)

Targeting Telomeres and Telomerase: Studies in Aging and Disease Utilizing CRISPR/Cas9 Technology
by: Andrew C. Brane, et al.
Published: (2019-02-01)

Allocation of Resources to Cyanogenic Glucosides Does Not Incur a Growth Sacrifice in <i>Sorghum bicolor</i> (L.) Moench
by: Muhammad N. Sohail, et al.
Published: (2020-12-01)

Optimization of highly efficient exogenous-DNA-free Cas9-ribonucleoprotein mediated gene editing in disease susceptibility loci in wheat (Triticum aestivum L.)
by: Snigdha Poddar, et al.
Published: (2023-01-01)

Efficient encapsulation of CRISPR-Cas9 RNP in bioreducible nanogels and release in a cytosol-mimicking environment
by: Peter Westarp, et al.
Published: (2025-07-01)

CRISPR-PN2: a flexible and genome-aware platform for diverse CRISPR experiments in parasitic nematodes
by: Damien M O'Halloran
Published: (2021-09-01)

Fundamental CRISPR-Cas9 tools and current applications in microbial systems
by: Pingfang Tian, et al.
Published: (2017-09-01)

Engineering CRISPR/Cas9 therapeutics for cancer precision medicine
by: Aditya Kumar Sharma, et al.
Published: (2024-04-01)

CRISPR-Cas9: A Powerful Tool to Efficiently Engineer <i>Saccharomyces cerevisiae</i>
by: João Rainha, et al.
Published: (2020-12-01)

MePHD1.2 affects the synthesis of cyanogenic glycosides by regulating transcription of MeCYP79D2 in cassava
by: Mengtao Li, et al.
Published: (2025-02-01)

Epigenome editing of the potential enhancers of the schizophrenia risk genes: approaches for optimization of the genetic constructs
by: D. A. Abashkin, et al.
Published: (2019-12-01)

CRISPR/Cas9 technology in the modeling of and treatment of mucopolysaccharidosis
by: Mehran Reyhani-Ardabili, et al.
Published: (2024-09-01)

Potensi kombinasi induced-pluripotent stem cells (iPSC) dan metode pengeditan gen clustered regularly interspaced short palindromic repeats–associated nuclease 9 (CRISPR-Cas9) sebagai terapi pada pasien hemofilia
by: Irfan Hasbullah Putra, et al.
Published: (2023-11-01)

CRISPR/Cas9 in Chronic Lymphocytic Leukemia
by: María Hernández-Sánchez
Published: (2022-05-01)

RNA-dependent RNA targeting by CRISPR-Cas9
by: Steven C Strutt, et al.
Published: (2018-01-01)

Homology-Directed-Repair-Based Genome Editing in HSPCs for the Treatment of Inborn Errors of Immunity and Blood Disorders
by: Daniel Allen, et al.
Published: (2023-04-01)

The Many (Inter)faces of Anti-CRISPRs: Modulation of CRISPR-Cas Structure and Dynamics by Mechanistically Diverse Inhibitors
by: Helen B. Belato, et al.
Published: (2023-01-01)

High-throughput sgRNA testing reveals rules for Cas9 specificity and DNA repair in tomato cells
by: Ellen Slaman, et al.
Published: (2023-06-01)

On the effect of fluid temperature in hydrodynamic cavitation on a chip reactors
by: Seyedreza Tebyani, et al.
Published: (2025-05-01)

CRISPR-Cas guide RNA indel analysis using CRISPResso2 with Nanopore sequencing data
by: Gus Rowan McFarlane, et al.
Published: (2024-07-01)

Emerging Gene-editing nano-therapeutics for Cancer
by: Najma Nujoom, et al.
Published: (2024-11-01)

New Insights into the Runt Domain of RUNX2 in Melanoma Cell Proliferation and Migration
by: Michela Deiana, et al.
Published: (2018-11-01)

An overview of applications of CRISPR-Cas technologies in biomedical engineering
by: Saleh Jamehdor, et al.
Published: (2020-10-01)

Targeting miRNA by CRISPR/Cas in cancer: advantages and challenges
by: Bashdar Mahmud Hussen, et al.
Published: (2023-07-01)

A Review on the Potential of Application of CRISPR/Cas9 Technology in CYP3A4 and CYP2D6 Genes: A Way to Move Forward in Identifying New Drug Targets
by: Fazleen Haslinda Mohd Hatta, et al.
Published: (2022-11-01)

Principles and medical applications of gene editing by CRISPR / Cas
by: Rolando Sánchez Artigas, et al.
Published: (2021-12-01)

CYP1B1 Knockout in a Bovine Hepatocyte-like Cell Line (BFH12) Unveils Its Role in Liver Homeostasis and Aflatoxin B1-Induced Hepatotoxicity
by: Silvia Iori, et al.
Published: (2025-06-01)

Harnessing an anti-CRISPR protein for powering CRISPR/Cas9-mediated genome editing in undomesticated Bacillus strains
by: Man Su Kim, et al.
Published: (2025-06-01)

On the Effect of the Respiratory Droplet Generation Condition on COVID-19 Transmission
by: Ali Hosseinpour Shafaghi, et al.
Published: (2020-07-01)

Immunomodulation by Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein 9
by: Duygu Kırkık, et al.
Published: (2025-03-01)

CRISPR-mediated modulation of EGFR signaling in lung cancer
by: Mobina Tabibian, et al.
Published: (2025-01-01)

Identifying in vivo genetic dependencies of melanocyte and melanoma development
by: Sarah Perlee, et al.
Published: (2025-08-01)

Nanotechnology-Based Delivery of CRISPR/Cas9 for Cancer Treatment: A Comprehensive Review
by: Mohd Ahmar Rauf, et al.
Published: (2025-07-01)

The CRISPR/Cas system: Gene Editing by bacterial defense
by: Amira H. El-Ashry
Published: (2023-09-01)

Perspectives in Genome-Editing Techniques for Livestock
by: Julia Popova, et al.
Published: (2023-08-01)