Isolation and screening of azo dye decolorizing bacterial isolates from dye-contaminated textile wastewater

• Published in: Soil & Environment
• Main Authors: Shahid Mahmood, Muhammad Arshad, Azeem Khalid, Zilli Huma Nazli, Tariq Mahmood
• Format: Article
• Language: English
• Published: Soil Science Society of Pakistan (SSSP) 2011-04-01
• Subjects: Azo dyes; Remazol Black-B; Wastewater; Yeast extract; Textile effluent
• Online Access: http://www.se.org.pk/File-Download.aspx?publishedid=23

Azo dyes are released into wastewater streams without any pretreatment and pollute water and soilenvironments. To prevent contamination of our vulnerable resources, removal of these dye pollutants is of greatimportance. For this purpose, wastewater samples were collected from dye-contaminated sites of Faisalabad. About200 bacterial isolates were isolated through enrichment and then tested for their potential to remove RemazolBlack-B azo dye in liquid medium. Five bacterial isolates capable of degrading Remazol Black-B azo dye efficientlywere screened through experimentation on modified mineral salt medium. Isolate SS1 (collected from wastewater ofSupreme Textile Industry) was able to completely remove the Remazol Black-B dye from the liquid medium in 18 h.Further, the isolate showed the best performance at the dye concentration of 100 mg L-1 medium (pH 7) and attemperature 35oC. Similarly, yeast extract proved to be the best carbon source for decolorization purpose. Theresults imply that the isolate SS1 could be used for the removal of the reactive dyes from textile effluents.