Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems.
Recombinant proteins are commonly expressed in eukaryotic expression systems to ensure the formation of disulfide bridges and proper glycosylation. Although many proteins can be expressed easily, some proteins, sub-domains, and mutant protein versions can cause problems. Here, we investigated expres...
| Published in: | PLoS ONE |
|---|---|
| Main Authors: | Raphael Reuten, Denise Nikodemus, Maria B Oliveira, Trushar R Patel, Bent Brachvogel, Isabelle Breloy, Jörg Stetefeld, Manuel Koch |
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Online Access: | http://europepmc.org/articles/PMC4814134?pdf=render |
Similar Items
Use of a 96-Well Format for the Affinity Purification of Maltose-Binding Protein (MBP) Fusion Proteins
by: Richard Felleisen, et al.
Published: (1996-04-01)
by: Richard Felleisen, et al.
Published: (1996-04-01)
Purity of maltose-binding protein – Recombinant streptavidin expressed in Escherichia coli BL21 (pD861-MBP: 327892)
by: Toto Subroto, et al.
Published: (2022-01-01)
by: Toto Subroto, et al.
Published: (2022-01-01)
Purification of FLAG-tagged Secreted Proteins from Mammalian Cells
by: Eisuke Itakura, et al.
Published: (2017-08-01)
by: Eisuke Itakura, et al.
Published: (2017-08-01)
Vectors for Expression and Secretion of FLAG Epitope-Tagged Proteins in Mammalian Cells
by: Richard G. Chubet, et al.
Published: (1996-01-01)
by: Richard G. Chubet, et al.
Published: (1996-01-01)
Protocol for detecting histidine polyphosphate modification of human proteins via MBP-tagged expression in E. coli
by: Kirsten Lehotsky, et al.
Published: (2024-06-01)
by: Kirsten Lehotsky, et al.
Published: (2024-06-01)
Dynamical Signatures of Thermotoga maritima Maltose-Binding Proteins Affected by Ligand Binding
by: Shantanu Shukla, et al.
Published: (2025-09-01)
by: Shantanu Shukla, et al.
Published: (2025-09-01)
Heparin Mimetics and Their Impact on Extracellular Matrix Protein Assemblies
by: Fabian Heide, et al.
Published: (2023-03-01)
by: Fabian Heide, et al.
Published: (2023-03-01)
Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides
by: Guido Tomás Rozenblum, et al.
Published: (2014-01-01)
by: Guido Tomás Rozenblum, et al.
Published: (2014-01-01)
Accessing a hidden conformation of the maltose binding protein using accelerated molecular dynamics.
by: Denis Bucher, et al.
Published: (2011-04-01)
by: Denis Bucher, et al.
Published: (2011-04-01)
Protocol for CRISPR-based endogenous protein tagging in mammalian cells
by: Yu-Xi Xiao, et al.
Published: (2024-12-01)
by: Yu-Xi Xiao, et al.
Published: (2024-12-01)
Protocol for CRISPR-based endogenous protein tagging in mammalian cells
by: Yu-Xi Xiao, et al.
Published: (2025-03-01)
by: Yu-Xi Xiao, et al.
Published: (2025-03-01)
Dual-tagging system for the affinity purification of mammalian protein complexes
by: Richard J. Giannone, et al.
Published: (2007-09-01)
by: Richard J. Giannone, et al.
Published: (2007-09-01)
Coupling of MBP fusion protein cleavage with sparse matrix crystallization screens to overcome problematic protein solubility
by: Franz Gruswitz, et al.
Published: (2005-10-01)
by: Franz Gruswitz, et al.
Published: (2005-10-01)
Presence and removal of a contaminating NADH oxidation activity in recombinant maltose-binding protein fusion proteins expressed in Escherichia coli
by: Fengguang Guo, et al.
Published: (2012-04-01)
by: Fengguang Guo, et al.
Published: (2012-04-01)
Soluble prokaryotic overexpression and purification of bioactive human granulocyte colony-stimulating factor by maltose binding protein and protein disulfide isomerase.
by: Bich Hang Do, et al.
Published: (2014-01-01)
by: Bich Hang Do, et al.
Published: (2014-01-01)
MBP Fusion Protein with a Viral Protease Cleavage Site: One-Step Cleavage/Purification of Insoluble Proteins
by: Andrei Alexandrov, et al.
Published: (2001-06-01)
by: Andrei Alexandrov, et al.
Published: (2001-06-01)
Mammalian Expression Vectors for Epitope Tag Fusion Proteins that Are Toxic in E. coli
by: Rolf Jakobi, et al.
Published: (2002-12-01)
by: Rolf Jakobi, et al.
Published: (2002-12-01)
Electron Microscopic Detection of Proteins and Protein Complexes in Mammalian Cells Using APEX-tagged, Conditionally Stable Nanobodies
by: Thomas Hall, et al.
Published: (2018-11-01)
by: Thomas Hall, et al.
Published: (2018-11-01)
Detection of Glu-Glu-Tagged Proteins in Mammalian Cell Culture Media by Dot Immunoblotting
by: Jeff L. Ellsworth, et al.
Published: (2000-05-01)
by: Jeff L. Ellsworth, et al.
Published: (2000-05-01)
Estimating Cellular Abundances of Halo-tagged Proteins in Live Mammalian Cells by Flow Cytometry
by: Claudia Cattoglio, et al.
Published: (2020-02-01)
by: Claudia Cattoglio, et al.
Published: (2020-02-01)
Effect of Cholesterol and Myelin Basic Protein (MBP) Content on Lipid Monolayers Mimicking the Cytoplasmic Membrane of Myelin
by: Jennica Träger, et al.
Published: (2020-02-01)
by: Jennica Träger, et al.
Published: (2020-02-01)
The lateral membrane organization and dynamics of myelin proteins PLP and MBP are dictated by distinct galactolipids and the extracellular matrix.
by: Hande Ozgen, et al.
Published: (2014-01-01)
by: Hande Ozgen, et al.
Published: (2014-01-01)
Highly Efficient Protein and Complex Purification from Mammalian Cells Using the HaloTag® Technology
by: Jacqui L. Méndez, et al.
Published: (2011-10-01)
by: Jacqui L. Méndez, et al.
Published: (2011-10-01)
Nonenzymatic Browning of Amorphous Maltose/Whey Protein Isolates Matrix: Effects of Water Sorption and Molecular Mobility
by: Yaowen Wu, et al.
Published: (2022-07-01)
by: Yaowen Wu, et al.
Published: (2022-07-01)
Production of recombinant HPV11/16 E6/E7-MBP-His6 fusion proteins and their potential to induce cytokine secretion by immune cells in peripheral blood
by: Mei-nian Xu, et al.
Published: (2024-01-01)
by: Mei-nian Xu, et al.
Published: (2024-01-01)
In silico designing of multiepitope-based-peptide (MBP) vaccine against MAPK protein express for Alzheimer's disease in Zebrafish
by: Yasir Arfat, et al.
Published: (2023-11-01)
by: Yasir Arfat, et al.
Published: (2023-11-01)
PA MBP - firm production, high quality
by: E. S. Ameliantchik
Published: (2000-05-01)
by: E. S. Ameliantchik
Published: (2000-05-01)
Regulation of Maltose Transport and Metabolism in Saccharomyces cerevisiae
by: Srđan Novak, et al.
Published: (2004-01-01)
by: Srđan Novak, et al.
Published: (2004-01-01)
Effects of Concentration of Soybean Protein Isolate and Maltose and Oil Phase Volume Fraction on Freeze–Thaw Stability of Pickering Emulsion
by: Ziyue Song, et al.
Published: (2022-12-01)
by: Ziyue Song, et al.
Published: (2022-12-01)
Deconvolution of the MBP-Bri2 Interaction by a Yeast Two Hybrid System and Synergy of the AlphaFold2 and High Ambiguity Driven Protein-Protein Docking
by: Evgeniya V. Smirnova, et al.
Published: (2022-01-01)
by: Evgeniya V. Smirnova, et al.
Published: (2022-01-01)
A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch–Agarose Mixture and Its Application for Convenient Protein–Protein Interaction Analysis
by: Yuri Emoto, et al.
Published: (2023-04-01)
by: Yuri Emoto, et al.
Published: (2023-04-01)
Methodology to Create Auxin-Inducible Degron Tagging System to Control Expression of a Target Protein in Mammalian Cell Lines
by: Amit Rahi, et al.
Published: (2024-01-01)
by: Amit Rahi, et al.
Published: (2024-01-01)
A Robust Expression and Purification Method for Production of <i>Sp</i>Cas9-GFP-MBP Fusion Protein for In Vitro Applications
by: Andrea Luciana Fleitas, et al.
Published: (2022-05-01)
by: Andrea Luciana Fleitas, et al.
Published: (2022-05-01)
EGF-Coupled Gold Nanoparticles Increase the Expression of CNPase and the Myelin-Associated Proteins MAG, MOG, and MBP in the Septal Nucleus Demyelinated by Cuprizone
by: Eduardo Lira-Diaz, et al.
Published: (2022-02-01)
by: Eduardo Lira-Diaz, et al.
Published: (2022-02-01)
Early Bioinformatic Implication of Triacidic Amino Acid Motifs in Autophagy-Dependent Unconventional Secretion of Mammalian Proteins
by: Malay Ranjan Biswal, et al.
Published: (2022-05-01)
by: Malay Ranjan Biswal, et al.
Published: (2022-05-01)
Aroma Potential of a New Maltose-Negative Yeast Isolate
by: Selin Yabacı Karaoğlan, et al.
Published: (2025-09-01)
by: Selin Yabacı Karaoğlan, et al.
Published: (2025-09-01)
Stoichiometric Conversion of Maltose for Biomanufacturing by In Vitro Synthetic Enzymatic Biosystems
by: Guowei Li, et al.
Published: (2022-01-01)
by: Guowei Li, et al.
Published: (2022-01-01)
Structural basis for negative regulation of the Escherichia coli maltose system
by: Yuang Wu, et al.
Published: (2023-08-01)
by: Yuang Wu, et al.
Published: (2023-08-01)
Utilization of maltose enriched spent grains liquor for xanthan production
by: Dodić Jelena M., et al.
Published: (2011-01-01)
by: Dodić Jelena M., et al.
Published: (2011-01-01)
Genome wide CRISPR screen for Pasteurella multocida toxin (PMT) binding proteins reveals LDL Receptor Related Protein 1 (LRP1) as crucial cellular receptor.
by: Julian Schoellkopf, et al.
Published: (2022-12-01)
by: Julian Schoellkopf, et al.
Published: (2022-12-01)
Similar Items
-
Use of a 96-Well Format for the Affinity Purification of Maltose-Binding Protein (MBP) Fusion Proteins
by: Richard Felleisen, et al.
Published: (1996-04-01) -
Purity of maltose-binding protein – Recombinant streptavidin expressed in Escherichia coli BL21 (pD861-MBP: 327892)
by: Toto Subroto, et al.
Published: (2022-01-01) -
Purification of FLAG-tagged Secreted Proteins from Mammalian Cells
by: Eisuke Itakura, et al.
Published: (2017-08-01) -
Vectors for Expression and Secretion of FLAG Epitope-Tagged Proteins in Mammalian Cells
by: Richard G. Chubet, et al.
Published: (1996-01-01) -
Protocol for detecting histidine polyphosphate modification of human proteins via MBP-tagged expression in E. coli
by: Kirsten Lehotsky, et al.
Published: (2024-06-01)
