Identification and reciprocal introgression of a QTL affecting body mass in mice

• 出版年: Genetics Selection Evolution
• 主要な著者: Pignatelli Pat M, Knott Sara A, Rance Kellie A, Christians Julian K, Oliver Fiona, Bünger Lutz
• フォーマット: 論文
• 言語: ドイツ語
• 出版事項: BMC 2004-09-01
• 主題: quantitative trait loci; introgression; epistasis; body mass
• オンライン･アクセス: http://www.gsejournal.org/content/36/5/577
• ISSN: 0999-193X; 1297-9686

<p>Abstract</p> <p>The aim of this study was to examine the effects of a QTL in different genetic backgrounds. A QTL affecting body mass on chromosome 6 was identified in an F₂cross between two lines of mice that have been divergently selected for this trait. The effect of the QTL on mass increased between 6 and 10 weeks of age and was not sex-specific. Body composition analysis showed effects on fat-free dry body mass and fat mass. To examine the effect of this QTL in different genetic backgrounds, the high body mass sixth chromosome was introgressed into the low body mass genetic background and <it>vice versa </it>by repeated marker-assisted backcrossing. After three generations of backcrossing, new F₂populations were established within each of the introgression lines by crossing individuals that were heterozygous across the sixth chromosome. The estimated additive effect of the QTL on 10-week body mass was similar in both genetic backgrounds and in the original F₂population (<it>i.e.</it>, ~0.4 phenotypic standard deviations); no evidence of epistatic interaction with the genetic background was found. The 95% confidence interval for the location of the QTL was refined to a region of approximately 7 cM between <it>D6Mit268 </it>and <it>D6Mit123</it>.</p>