Inhibition of Klf10 Attenuates Oxidative Stress-Induced Senescence of Chondrocytes via Modulating Mitophagy

• Published in: Molecules
• Main Authors: Jie Shang, Nan Lin, Rong Peng, Ning Jiang, Biao Wu, Baizhou Xing, Shiyuan Lin, Xianghe Xu, Huading Lu
• Format: Article
• Language: English
• Published: MDPI AG 2023-01-01
• Subjects: osteoarthritis; Klf10; senescence; mitochondria; autophagy
• Online Access: https://www.mdpi.com/1420-3049/28/3/924

Osteoarthritis (OA) is the most prevalent degenerative joint disease in the elderly. Accumulation of evidence has suggested that chondrocyte senescence plays a significant role in OA development. Here, we show that Krüppel-like factor 10 (Klf10), also named TGFβ inducible early gene-1 (TIEG1), is involved in the pathology of chondrocyte senescence. Knocking down the Klf10 in chondrocytes attenuated the tert-butyl hydroperoxide (TBHP)-induced senescence, inhibited generation of reactive oxygen species (ROS), and maintained mitochondrial homeostasis by activating mitophagy. These findings suggested that knocking down Klf10 inhibited senescence-related changes in chondrocytes and improved cartilage homeostasis, indicating that Klf10 may be a therapeutic target for protecting cartilage against OA.