Protocols for the preparation and characterization of decellularized tissue and organ scaffolds for tissue engineering

• 出版年: BioTechniques
• 主要な著者: Dar-Jen Hsieh, Periasamy Srinivasan, Ko-Chung Yen, Yi-Chun Yeh, Yun-Ju Chen, Hung-Chou Wang, Yih-Wen Tarng
• フォーマット: 論文
• 言語: 英語
• 出版事項: Taylor & Francis Group 2021-02-01
• 主題: 3D bioprinting; decellularized tissue scaffolds; organ regeneration; organ scaffolds; porcine organs; porcine tissues
• オンライン･アクセス: https://www.future-science.com/doi/10.2144/btn-2020-0141
• ISSN: 0736-6205; 1940-9818

Extracellular matrix (ECM) scaffolds are extensively used in tissue engineering studies and numerous clinical applications for tissue and organ reconstructions. Due to the global severe shortage of human tissues and organs, xenogeneic biomaterials are a common source for human tissue engineering and regenerative medicine applications. Traditional methods for decellularization often disrupt the 3D architecture and damage the structural integrity of the ECM scaffold. To efficiently obtain natural ECM scaffolds from animal tissues and organs with intact architecture, we have developed a platform decellularization process using supercritical CO2 and tested its potential application in tissue engineering. A combination of human mesenchymal stem cells with a decellularized dermal matrix scaffold allowed complete regeneration of skin structure in a porcine full-thickness wound model.