Protocol for processing and analyzing multiplexed images improves lymphatic cell identification and spatial architecture in human tissue

Summary: Multiplexed images of human lymphatic tissue are extensively preprocessed before cell phenotyping and spatial analysis. Here, we present KINTSUGI (knowledge integration with new technologies: simplified user-guided image processing), a protocol designed to interactively engage the user in e...

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Bibliographic Details
Published in:	STAR Protocols
Main Authors:	Justin A. Smith, Drew T. Bloss, MacKenzie D. Williams, Amanda L. Posgai, Todd M. Brusko, Mark A. Atkinson, Clive H. Wasserfall, Maigan A. Brusko
Format:	Article
Language:	English
Published:	Elsevier 2025-09-01
Subjects:	Immunology Microscopy Protein Biochemistry Proteomics Computer sciences
Online Access:	http://www.sciencedirect.com/science/article/pii/S266616672500382X

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