Generation of Brachyury-mCherry knock-in reporter human pluripotent stem cell line (SNUe003-A-2) using CRISPR/CAS9 nuclease

Brachyury is an embryonic nuclear transcription factor required for mesoderm formation and differentiation. Here, we introduced an mCherry reporter into the C-terminus of Brachyury in the human pluripotent stem cell line SNUhES3 using the CRISPR/Cas9 nuclease approach. Successful gene editing was ve...

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التفاصيل البيبلوغرافية
الحاوية / القاعدة:Stem Cell Research
المؤلفون الرئيسيون: Juwon Jung, Sunsook Hwang, Hyewon Seol, A-Hyeon Kim, Ha Myoung Lee, Jin Jea Sung, Seung Min Jeong, Young Min Choi, Jong Kwan Jun, Dong-wook Kim, Jiho Jang
التنسيق: مقال
اللغة:الإنجليزية
منشور في: Elsevier 2021-05-01
الوصول للمادة أونلاين:http://www.sciencedirect.com/science/article/pii/S1873506121001677
الوصف
الملخص:Brachyury is an embryonic nuclear transcription factor required for mesoderm formation and differentiation. Here, we introduced an mCherry reporter into the C-terminus of Brachyury in the human pluripotent stem cell line SNUhES3 using the CRISPR/Cas9 nuclease approach. Successful gene editing was verified by DNA sequencing. SNUhES3-Brachyury-mCherry cells expressed pluripotent stem cell markers, exhibited a normal karyotype, and could generate all three germ layers. This cell line expressed the red fluorescence protein mCherry upon the induction of mesoderm differentiation. This reporter cell line could be used to monitor mesodermal population enrichment during mesodermal differentiation.
تدمد:1873-5061

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