Clitorea ternatea flower extract induces platelet-derived growth factor (PDGF) and GPx gene overexpression in ultraviolet (UV) B irradiationinduced collagen loss

• Published in: Medicinski Glasnik
• Main Authors: Rizka Sofyanti Putri, Agung Putra, Chodidjah, Dewi Masyitah Darlan, Setyo Trisnadi, Siti Thomas, Nur Dina Amalina, Rizky Candra Irawan
• Format: Article
• Language: English
• Published: Medical Association of Zenica-Doboj Canton 2023-02-01
• Subjects: clitorea ternatea flower extract; collagen loss; gpx; pdgf
• Online Access: https://ljkzedo.ba/mgpdf/mg38/15_Putri_1530_A.pdf
• ISSN: 1840-0132; 1840-2445

Aim To determine the effect of Clitorea ternatea flower extract (CTFE) in the gel dosage form on the expression of GPx and platelet-derived growth factor (PDGF) in ultraviolet (UV) - B irradiation-induced collagen loss rat model. Methods This is experimental research with post-test control group design. Twenty healthy male Wistar rats were divided into four treatment groups: a sham group, UVB control group, two treatment groups with gel of CTFE 5% and gel of CTFE 10%, respectively. Each group was treated with UVB at 302 nm with a MED of 160 mJ/cm2 for 5 days, whereas the sham group did not receive UVB. In the treatment groups CTFE 5% and CTFE 10% gel were given on the 6th to the 14th day. On day 14 all treatment groups were terminated, and GPx and PDGF gene expression were analysed using qRT-PCR. Results In the group of gel of CTFE 10%, there was a significant increase in GPx gene expression (9.51±1.83) and PDGF (4.36±1.18) compared to the UVB control group which had GPx and PDGF gene expression of 4.90±1.64) and 0.032±0.01, respectively. Conclusion The administration of CTFE gel showed an increase of the expression of GPx and PDGF genes on UVB irradiationinduced collagen loss rat model.