| Summary: | The natural populations of lactic acid bacteria (LAB) and silage quality of pineapple residue silage were investigated in this study. A total of 34 LAB strains originally isolated from pineapple residue silage were characterized and identified by phenotypic and genotypic methods. These LAB strains were Gram-positive and catalase-negative bacteria, which were divided into four groups: <i>Lactiplantibacillus</i><i>plantarum</i> (52.9%), <i>Levilactobacillus brevis</i> (14.7%), <i>Lacticaseibacillus paracasei</i> (17.6%) and <i>Leuconostoc citreum</i> (14.7%). <i>Lactiplantibacillus plantarum</i> was the dominant species. Homofermentative strains accounted for 70.5%. After 30 days of ensiling, the pineapple residue silage could be well preserved with low pH value (3.65) and high content of lactic acid (75.57 g/kg of DM). In this study, LAB populations of pineapple residue silage fermentation were investigated, which indicated that pineapple residue silage was a potential good animal feed source. In addition, this result will be valuable for screening-appropriate inoculants aimed at improving the quality.
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