| Summary: | Moringa oleifera Lam. is a member of the Moringaceae family which includes 13 species. The plant is cultivated throughout tropical and subtropical areas due to its adaptability, also used worldwide as nutritional supplement and traditional medicine. In this study, the complete chloroplast genome (cp) of M. oleifera was sequenced, assembled, and analyzed. Our results indicated that the cp genome of M. oleifera was 160,600 bp in length, containing a large single copy (LSC, 88,727 bp) region and a small single copy (SSC, 18,883 bp) region which separated by two reduced inverted repeats (IRs, 26,495 bp). A total of 108 unique genes were identified, including 74 protein-coding genes, 30 transfer RNA (tRNA) genes, and four ribosomal RNA (rRNA) genes. The overall GC content of M. oleifera cp genome was 36.8%. Most of the 47 simple sequence repeats (SSRs) were mononucleotides motifs of A/T types and were found to be located in non-coding regions. Phylogenetic analysis of M. oleifera showed that M. oleifera was the base group of Rhoeadales with strongly bootstrap supported which is consist with morphological taxonomy.
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