Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota Fungi
Calcium controls important processes in fungal metabolism, such as hyphae growth, cell wall synthesis, and stress tolerance. Recently, it was reported that calcium affects polyphosphate and lipid accumulation in fungi. The purpose of this study was to assess the effect of calcium on the accumulation...
| الحاوية / القاعدة: | Journal of Fungi |
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| المؤلفون الرئيسيون: | , , , , , , , , |
| التنسيق: | مقال |
| اللغة: | الإنجليزية |
| منشور في: |
MDPI AG
2021-04-01
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://www.mdpi.com/2309-608X/7/4/300 |
| _version_ | 1850310033803313152 |
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| author | Simona Dzurendova Boris Zimmermann Achim Kohler Kasper Reitzel Ulla Gro Nielsen Benjamin Xavier Dupuy--Galet Shaun Leivers Svein Jarle Horn Volha Shapaval |
| author_facet | Simona Dzurendova Boris Zimmermann Achim Kohler Kasper Reitzel Ulla Gro Nielsen Benjamin Xavier Dupuy--Galet Shaun Leivers Svein Jarle Horn Volha Shapaval |
| author_sort | Simona Dzurendova |
| collection | DOAJ |
| container_title | Journal of Fungi |
| description | Calcium controls important processes in fungal metabolism, such as hyphae growth, cell wall synthesis, and stress tolerance. Recently, it was reported that calcium affects polyphosphate and lipid accumulation in fungi. The purpose of this study was to assess the effect of calcium on the accumulation of lipids and polyphosphate for six oleaginous Mucoromycota fungi grown under different phosphorus/pH conditions. A Duetz microtiter plate system (Duetz MTPS) was used for the cultivation. The compositional profile of the microbial biomass was recorded using Fourier-transform infrared spectroscopy, the high throughput screening extension (FTIR-HTS). Lipid content and fatty acid profiles were determined using gas chromatography (GC). Cellular phosphorus was determined using assay-based UV-Vis spectroscopy, and accumulated phosphates were characterized using solid-state <sup>31</sup>P nuclear magnetic resonance spectroscopy. Glucose consumption was estimated by FTIR-attenuated total reflection (FTIR-ATR). Overall, the data indicated that calcium availability enhances polyphosphate accumulation in Mucoromycota fungi, while calcium deficiency increases lipid production, especially under acidic conditions (pH 2–3) caused by the phosphorus limitation. In addition, it was observed that under acidic conditions, calcium deficiency leads to increase in carotenoid production. It can be concluded that calcium availability can be used as an optimization parameter in fungal fermentation processes to enhance the production of lipids or polyphosphates. |
| format | Article |
| id | doaj-art-94de89b3a9d74297b15451df575547c9 |
| institution | Directory of Open Access Journals |
| issn | 2309-608X |
| language | English |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-94de89b3a9d74297b15451df575547c92025-08-19T23:27:27ZengMDPI AGJournal of Fungi2309-608X2021-04-017430010.3390/jof7040300Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota FungiSimona Dzurendova0Boris Zimmermann1Achim Kohler2Kasper Reitzel3Ulla Gro Nielsen4Benjamin Xavier Dupuy--Galet5Shaun Leivers6Svein Jarle Horn7Volha Shapaval8Faculty of Science and Technology, Norwegian University of Life Sciences, Drøbakveien 31, 1433 Ås, NorwayFaculty of Science and Technology, Norwegian University of Life Sciences, Drøbakveien 31, 1433 Ås, NorwayFaculty of Science and Technology, Norwegian University of Life Sciences, Drøbakveien 31, 1433 Ås, NorwayDepartment of Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, DenmarkDepartment of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, DenmarkFaculty of Science and Technology, Norwegian University of Life Sciences, Drøbakveien 31, 1433 Ås, NorwayFaculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Christian Magnus Falsens vei 1, 1433 Ås, NorwayFaculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Christian Magnus Falsens vei 1, 1433 Ås, NorwayFaculty of Science and Technology, Norwegian University of Life Sciences, Drøbakveien 31, 1433 Ås, NorwayCalcium controls important processes in fungal metabolism, such as hyphae growth, cell wall synthesis, and stress tolerance. Recently, it was reported that calcium affects polyphosphate and lipid accumulation in fungi. The purpose of this study was to assess the effect of calcium on the accumulation of lipids and polyphosphate for six oleaginous Mucoromycota fungi grown under different phosphorus/pH conditions. A Duetz microtiter plate system (Duetz MTPS) was used for the cultivation. The compositional profile of the microbial biomass was recorded using Fourier-transform infrared spectroscopy, the high throughput screening extension (FTIR-HTS). Lipid content and fatty acid profiles were determined using gas chromatography (GC). Cellular phosphorus was determined using assay-based UV-Vis spectroscopy, and accumulated phosphates were characterized using solid-state <sup>31</sup>P nuclear magnetic resonance spectroscopy. Glucose consumption was estimated by FTIR-attenuated total reflection (FTIR-ATR). Overall, the data indicated that calcium availability enhances polyphosphate accumulation in Mucoromycota fungi, while calcium deficiency increases lipid production, especially under acidic conditions (pH 2–3) caused by the phosphorus limitation. In addition, it was observed that under acidic conditions, calcium deficiency leads to increase in carotenoid production. It can be concluded that calcium availability can be used as an optimization parameter in fungal fermentation processes to enhance the production of lipids or polyphosphates.https://www.mdpi.com/2309-608X/7/4/300Mucoromycotacalciumlipidspolyphosphatescarotenoidsbiorefinery |
| spellingShingle | Simona Dzurendova Boris Zimmermann Achim Kohler Kasper Reitzel Ulla Gro Nielsen Benjamin Xavier Dupuy--Galet Shaun Leivers Svein Jarle Horn Volha Shapaval Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota Fungi Mucoromycota calcium lipids polyphosphates carotenoids biorefinery |
| title | Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota Fungi |
| title_full | Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota Fungi |
| title_fullStr | Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota Fungi |
| title_full_unstemmed | Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota Fungi |
| title_short | Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota Fungi |
| title_sort | calcium affects polyphosphate and lipid accumulation in mucoromycota fungi |
| topic | Mucoromycota calcium lipids polyphosphates carotenoids biorefinery |
| url | https://www.mdpi.com/2309-608X/7/4/300 |
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