Maintenance of mouse trophoblast stem cells in KSR-based medium allows conventional 3D culture

Mouse trophoblast stem cells (TSCs) can differentiate into trophoblast cells, which constitute the placenta. Under conventional culture conditions, in a medium supplemented with 20% fetal bovine serum (FBS), fibroblast growth factor 4 (FGF4), and heparin and in the presence of mouse embryonic fibrob...

Full description

Bibliographic Details
Published in:	The Journal of Reproduction and Development
Main Authors:	Shuai SUN, Shota YANO, Momo O NAKANISHI, Michiko HIROSE, Kazuhiko NAKABAYASHI, Kenichiro HATA, Atsuo OGURA, Satoshi TANAKA
Format:	Article
Language:	English
Published:	The Society for Reproduction and Development 2021-03-01
Subjects:	extracellular matrix knockouttm serum replacement suspension culture trophoblast stem cell
Online Access:	https://www.jstage.jst.go.jp/article/jrd/67/3/67_2020-119/_pdf/-char/en

Similar Items

Aberrant imprinting in mouse trophoblast stem cells established from somatic cell nuclear transfer-derived embryos
by: Michiko Hirose, et al.
Published: (2018-07-01)

Cross‐Species Insights into Trophoblast Invasion During Placentation Governed by Immune‐Featured Trophoblast Cells
by: Xupeng Zang, et al.
Published: (2024-11-01)

Persistent trophoblastic atypia on endocervical curettage found to be an epithelioid trophoblastic Tumor: A case report and review of the literature
by: Allison L. Brodsky, et al.
Published: (2022-06-01)

Persistent trophoblastic tumors
by: M. N. Tikhonovskaya, et al.
Published: (2014-07-01)

In Vitro Model of Human Trophoblast in Early Placentation
by: Darina Bačenková, et al.
Published: (2022-04-01)

Trophoblastic disease
by: L. A. Meshcheryakova
Published: (2015-01-01)

Epithelioid trophoblastic tumor of uterus presenting as an ovarian mass: A diagnostic and therapeutic dilemma
by: Shet Tanuja, et al.
Published: (2008-04-01)

Diagnostic challenges in cornual epithelioid trophoblastic tumor
by: Feng-Ying Fang, et al.
Published: (2014-06-01)

The role of DNA methylation in human trophoblast differentiation
by: Teena K. J. B. Gamage, et al.
Published: (2018-12-01)

GESTATIONAL TROPHOBLASTIC DISEASE: CLASSIFICATION, EPIDEMIOLOGY AND PATHOGENESIS
by: A. G. Solopova, et al.
Published: (2018-05-01)

Placental-site trophoblastic tumor: A case report
by: Liqin Gu, et al.
Published: (2025-05-01)

Nonmetastatic gestational trophoblastic tumor: 12 years experience in Songklanagarind Hospital
by: S Tocharoenvanich, et al.
Published: (2003-03-01)

Epigenetic Dysregulation of Trophoblastic Gene Expression in Gestational Trophoblastic Disease
by: Zoltan Szabolcsi, et al.
Published: (2021-12-01)

Single-cell characterization of self-renewing primary trophoblast organoids as modeling of EVT differentiation and interactions with decidual natural killer cells
by: Bai-Mei Zhuang, et al.
Published: (2023-10-01)

Placental site trophoblastic tumor – A rare gestational trophoblastic disease: A case report and review of literature
by: Prachi, et al.
Published: (2024-01-01)

Gestational trophoblastic disease: risk factors, current methods of diagnostics and treatment
by: A. G. Solopova, et al.
Published: (2018-08-01)

Is There a Role for Surgery in the Management of Gestational Trophoblastic Neoplasia?
by: Malihe Hasanzadeh, et al.
Published: (2022-01-01)

Epithelioid trophoblastic tumor
by: Sevinc Hallac Keser, et al.
Published: (2015-10-01)

Gestational trophoblastic neoplasia with pancreatic metastasis: clinical characteristics, treatment strategies, and outcomes
by: Xinghan Cheng, et al.
Published: (2025-09-01)

Stem Cell-Based Trophoblast Models to Unravel the Genetic Causes of Human Miscarriages
by: Tatiana V. Nikitina, et al.
Published: (2022-06-01)

Epithelioid Trophoblastic Tumor—A Challenge to Manage due to Its Rare Existence: A Case Report with Review of Literature
by: Neetha Nandan, et al.
Published: (2024-12-01)

Exploring maternal-fetal interface with in vitro placental and trophoblastic models
by: Xinlu Liu, et al.
Published: (2023-11-01)

Successful treatment of high-risk gestational trophoblastic disease in a patient with intolerance to standard chemotherapy (clinical case)
by: G. M. Teletaeva, et al.
Published: (2021-01-01)

Laparoscopic hysterectomy as a treatment modality for gestational trophoblastic neoplasia: A report of two cases
by: Jokanović Predrag, et al.
Published: (2022-01-01)

Gestational Trophoblastic Disease: Diagnostic and Therapeutic Updates in Light of Recent Evidence: A Literature Review
by: Giuseppe Gullo, et al.
Published: (2025-09-01)

Epithelioid trophoblastic tumor: Clinical presentation, diagnosis, and treatment
by: D. A. Lisayev, et al.
Published: (2014-08-01)

Successful management of stage IV epithelioid trophoblastic tumor using multimodality treatment: A case report
by: Brad Nakamura, et al.
Published: (2021-08-01)

Treatment outcomes and the role of surgery in gestational trophoblastic neoplasia: a population-based cohort study
by: Agnes Larsson, et al.
Published: (2025-06-01)

Primary epithelioid trophoblastic tumor of the lung: A case report
by: Qiongying Zhang, et al.
Published: (2024-04-01)

Epithelioid trophoblastic tumor of the uterus: currently available diagnostic methods, determining an effective treatment strategy (case report)
by: S. N. Mamedli, et al.
Published: (2019-11-01)

Is Doppler ultrasound useful for evaluating gestational trophoblastic disease?
by: Lawrence H. Lin, et al.

Platinum preparations in the treatment of trophoblastic tumors
by: I. V. Markina, et al.
Published: (2014-08-01)

HMGA1 Is a Potential Driver of Preeclampsia Pathogenesis by Interference with Extravillous Trophoblasts Invasion
by: Keiichi Matsubara, et al.
Published: (2021-05-01)

Demographics of epithelioid trophoblastic tumour and placental site trophoblastic tumour: a 21 year UK population study
by: P. Savage, et al.
Published: (2025-12-01)

Gestational trophoblastic disease: understanding the molecular mechanisms of placental tumours
by: Alina Nicheperovich, et al.
Published: (2025-01-01)

Placental site trophoblastic disease in practice of gynecologist (case report).
by: Igor E. Fadeev, et al.
Published: (2024-09-01)

Trophoblast Organoids: Capturing the Complexity of Early Placental Development In Vitro
by: Brady M. Wessel, et al.
Published: (2024-08-01)

A Rare Case of Epithelioid Trophoblastic Tumor Presenting as Hematoma of a Caesarean Scar in the Lower Uterine Segment
by: Radomir Aničić, et al.
Published: (2021-12-01)

Assessment of risk factors associated with post-molar gestational trophoblastic neoplasia: a retrospective cohort
by: Silvia Regina Piazzetta, et al.
Published: (2024-11-01)

Placental site trophoblastic tumour mimicking an intramural pregnancy: a case report and review of the literature
by: Angel Yordanov, et al.
Published: (2022-06-01)