Protocol for muscle fiber type and cross-sectional area analysis in cryosections of whole lower mouse hindlimbs

Summary: We outline a protocol to visualize all mouse lower hindlimb skeletal muscles simultaneously. We describe procedures for orientating the whole lower hindlimb in gum tragacanth prior to freezing, simplifying the proceeding experimental steps, and enhancing the comprehensiveness of characteriz...

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Bibliographic Details
Published in:	STAR Protocols
Main Authors:	Edmund Battey, Matthieu Dos Santos, Dipsikha Biswas, Pascal Maire, Kei Sakamoto
Format:	Article
Language:	English
Published:	Elsevier 2024-12-01
Subjects:	Cell Biology Cell isolation Cell separation/fractionation Metabolism Microscopy Model Organisms
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724005896

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