Low Doses of Anatase and Rutile Nanoparticles Differently Modulate Photosynthesis and Regulatory Genes: A Contribution to the Nanoagroindustry
Industrial applications of titanium dioxide nanoparticles (TiO<sub>2</sub> NPs) are wide, and their use in nano-fertilizing technology has been proposed in the last few years. Bioactivity evaluation of different TiO<sub>2</sub> NPs formulations is therefore crucial, not only...
| الحاوية / القاعدة: | Agriculture |
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| المؤلفون الرئيسيون: | , , , , , , , , , , , , |
| التنسيق: | مقال |
| اللغة: | الإنجليزية |
| منشور في: |
MDPI AG
2022-01-01
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://www.mdpi.com/2077-0472/12/2/190 |
| _version_ | 1850758876859727872 |
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| author | Nuno Mariz-Ponte Sara Sario Rafael J. Mendes Márcio Couto Emil Gimranov Marino Santos Cristiana V. Correia Anicia Gomes Paulo R. Oliveira-Pinto Isabel Amorim Maria Celeste Dias José Miguel P. Ferreira de Oliveira Conceição Santos |
| author_facet | Nuno Mariz-Ponte Sara Sario Rafael J. Mendes Márcio Couto Emil Gimranov Marino Santos Cristiana V. Correia Anicia Gomes Paulo R. Oliveira-Pinto Isabel Amorim Maria Celeste Dias José Miguel P. Ferreira de Oliveira Conceição Santos |
| author_sort | Nuno Mariz-Ponte |
| collection | DOAJ |
| container_title | Agriculture |
| description | Industrial applications of titanium dioxide nanoparticles (TiO<sub>2</sub> NPs) are wide, and their use in nano-fertilizing technology has been proposed in the last few years. Bioactivity evaluation of different TiO<sub>2</sub> NPs formulations is therefore crucial, not only to select the most appropriate formulation but also to validate potential agro-applications. In the current work, we compared the bioactivity of the two most used TiO<sub>2</sub> NPs formulations (anatase and rutile–anatase) on the photosynthesis of <i>Lactuca sativa</i>. Seeds were exposed to concentrations of 0, 10, and 50 mg L<sup>−1</sup> of anatase (A) or rutile–anatase (RA). Germination rate was not affected by NPs, but root growth was stimulated mainly by RA50. Compared with control, RA showed positive effects on photophosphorylation-related parameters. A50 was more efficient in promoting the gas exchange phase (PN, Ci, gs, and E) and in stimulating the absorption of some nutrients. Expanding on the biochemical and physiological data, we show that RA50 stimulated several genes coding for proteins involved in the electron transport in thylakoids (<i>psbA</i>, <i>petB</i>, <i>petA</i>, <i>psaA</i>, <i>psaC</i>, <i>ndhA</i>, <i>ndhD</i>) and ATP synthesis (<i>atpA</i>, <i>atpB</i>). The transcript coding for the large subunit of RuBisCO (<i>rbcL</i>), was stimulated by lower concentration (RA10). This suggests that RuBisCO is highly sensitive to these NPs even at low doses. RA at low doses has been demonstrated to be the most promising NP. These discriminative effects of TiO<sub>2</sub> NPs, based on their formulation and dose, may present advantages for their use in the precision nanoagroindustry. |
| format | Article |
| id | doaj-art-9a91eaef7ecd44aebb0624d2ae4c7571 |
| institution | Directory of Open Access Journals |
| issn | 2077-0472 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-9a91eaef7ecd44aebb0624d2ae4c75712025-08-19T22:34:31ZengMDPI AGAgriculture2077-04722022-01-0112219010.3390/agriculture12020190Low Doses of Anatase and Rutile Nanoparticles Differently Modulate Photosynthesis and Regulatory Genes: A Contribution to the NanoagroindustryNuno Mariz-Ponte0Sara Sario1Rafael J. Mendes2Márcio Couto3Emil Gimranov4Marino Santos5Cristiana V. Correia6Anicia Gomes7Paulo R. Oliveira-Pinto8Isabel Amorim9Maria Celeste Dias10José Miguel P. Ferreira de Oliveira11Conceição Santos12Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalFaculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalFaculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalFaculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalFaculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalFaculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalFaculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalFaculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalFaculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalFaculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalLAQV-REQUIMTE, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalLAQV-REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, R. Jorge de Viterbo Ferreira 228, 4050-313 Porto, PortugalFaculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, PortugalIndustrial applications of titanium dioxide nanoparticles (TiO<sub>2</sub> NPs) are wide, and their use in nano-fertilizing technology has been proposed in the last few years. Bioactivity evaluation of different TiO<sub>2</sub> NPs formulations is therefore crucial, not only to select the most appropriate formulation but also to validate potential agro-applications. In the current work, we compared the bioactivity of the two most used TiO<sub>2</sub> NPs formulations (anatase and rutile–anatase) on the photosynthesis of <i>Lactuca sativa</i>. Seeds were exposed to concentrations of 0, 10, and 50 mg L<sup>−1</sup> of anatase (A) or rutile–anatase (RA). Germination rate was not affected by NPs, but root growth was stimulated mainly by RA50. Compared with control, RA showed positive effects on photophosphorylation-related parameters. A50 was more efficient in promoting the gas exchange phase (PN, Ci, gs, and E) and in stimulating the absorption of some nutrients. Expanding on the biochemical and physiological data, we show that RA50 stimulated several genes coding for proteins involved in the electron transport in thylakoids (<i>psbA</i>, <i>petB</i>, <i>petA</i>, <i>psaA</i>, <i>psaC</i>, <i>ndhA</i>, <i>ndhD</i>) and ATP synthesis (<i>atpA</i>, <i>atpB</i>). The transcript coding for the large subunit of RuBisCO (<i>rbcL</i>), was stimulated by lower concentration (RA10). This suggests that RuBisCO is highly sensitive to these NPs even at low doses. RA at low doses has been demonstrated to be the most promising NP. These discriminative effects of TiO<sub>2</sub> NPs, based on their formulation and dose, may present advantages for their use in the precision nanoagroindustry.https://www.mdpi.com/2077-0472/12/2/190bioactivitycrop growthgene regulationphotosynthesistitanium-dioxide nanoparticles |
| spellingShingle | Nuno Mariz-Ponte Sara Sario Rafael J. Mendes Márcio Couto Emil Gimranov Marino Santos Cristiana V. Correia Anicia Gomes Paulo R. Oliveira-Pinto Isabel Amorim Maria Celeste Dias José Miguel P. Ferreira de Oliveira Conceição Santos Low Doses of Anatase and Rutile Nanoparticles Differently Modulate Photosynthesis and Regulatory Genes: A Contribution to the Nanoagroindustry bioactivity crop growth gene regulation photosynthesis titanium-dioxide nanoparticles |
| title | Low Doses of Anatase and Rutile Nanoparticles Differently Modulate Photosynthesis and Regulatory Genes: A Contribution to the Nanoagroindustry |
| title_full | Low Doses of Anatase and Rutile Nanoparticles Differently Modulate Photosynthesis and Regulatory Genes: A Contribution to the Nanoagroindustry |
| title_fullStr | Low Doses of Anatase and Rutile Nanoparticles Differently Modulate Photosynthesis and Regulatory Genes: A Contribution to the Nanoagroindustry |
| title_full_unstemmed | Low Doses of Anatase and Rutile Nanoparticles Differently Modulate Photosynthesis and Regulatory Genes: A Contribution to the Nanoagroindustry |
| title_short | Low Doses of Anatase and Rutile Nanoparticles Differently Modulate Photosynthesis and Regulatory Genes: A Contribution to the Nanoagroindustry |
| title_sort | low doses of anatase and rutile nanoparticles differently modulate photosynthesis and regulatory genes a contribution to the nanoagroindustry |
| topic | bioactivity crop growth gene regulation photosynthesis titanium-dioxide nanoparticles |
| url | https://www.mdpi.com/2077-0472/12/2/190 |
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