| Summary: | Low temperatures are the most significant abiotic stressor for the conservation and production of <i>Phalaenopsis</i> in non-tropical areas. CBF/DREB1 transcription factors play an important role in the plant abiotic stress response. In this study, 31 DREB family members were identified in the <i>Phalaenopsis</i> genome. Expression pattern analysis showed that the expression of different <i>PaDREB</i> members varied among tissue sites. <i>PaDREB1D</i> was isolated from <i>Phalaenopsis aphrodite</i>, and multiple sequence alignment showed that <i>PaDREB1D</i> belonged to the A1 subgroup of the DREB family and was localized in the nucleus. <i>PaDREB1D</i> overexpression in protocorm-like bodies of <i>Phalaenopsis</i> reduced cell damage during low-temperature stress, increased antioxidant enzyme activity, and enhanced the low-temperature tolerance of protocorm-like bodies. The results of this study provide a theoretical basis for breeding for cold resistance and investigating the molecular mechanisms related to low-temperature responses in <i>Phalaenopsis</i>.
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