Optimizing the CRISPR/Cas9 system for gene editing in Yarrowia lipolytica

Yarrowia lipolytica is a promising host for producing valuable chemicals owing to its robustness and metabolic versatility. Efficient genome editing tools are essential for advancing its biotechnological applications. Although CRISPR/Cas9 technology has been applied in Y. lipolytica, achieving a con...

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Bibliographic Details

Published in:	Engineering Microbiology
Main Authors:	Jianhui Liu, Yamin Zhu, Jin Hou
Format:	Article
Language:	English
Published:	Elsevier 2025-06-01
Subjects:	CRISPR/Cas9 technology Yarrowia lipolytica sgRNA promoter Cas9 expression strategy Multiplex gene editing Genome integration
Online Access:	http://www.sciencedirect.com/science/article/pii/S2667370325000050