Generation of beta actin reporter line (BJNhem20 ACTB-eGFP) in human embryonic stem cells BJNhem20 using CRISPR-Cas9 gene targeting
Beta actin is a cytoskeletal protein that contributes to a wide range of cellular processes. Here we generated beta actin reporter knock-in in BJNhem20 human embryonic stem cell line by CRISPR Cas9 gene editing. The reporter mEGFP is integrated at the beta actin locus, tagging the N-terminal of the...
| Published in: | Stem Cell Research |
|---|---|
| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506125001333 |
| _version_ | 1849328168389836800 |
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| author | Pratiksha P. Bhat Maneesha S. Inamdar |
| author_facet | Pratiksha P. Bhat Maneesha S. Inamdar |
| author_sort | Pratiksha P. Bhat |
| collection | DOAJ |
| container_title | Stem Cell Research |
| description | Beta actin is a cytoskeletal protein that contributes to a wide range of cellular processes. Here we generated beta actin reporter knock-in in BJNhem20 human embryonic stem cell line by CRISPR Cas9 gene editing. The reporter mEGFP is integrated at the beta actin locus, tagging the N-terminal of the protein via a linker. The reporter line is a valuable tool to study beta actin dynamics during cellular process in human embryonic stem cells and to track cells by live imaging. |
| format | Article |
| id | doaj-art-a67d22e63b1441b695a8d5ba6dec7df8 |
| institution | Directory of Open Access Journals |
| issn | 1873-5061 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| spelling | doaj-art-a67d22e63b1441b695a8d5ba6dec7df82025-08-30T07:26:06ZengElsevierStem Cell Research1873-50612025-09-018710378310.1016/j.scr.2025.103783Generation of beta actin reporter line (BJNhem20 ACTB-eGFP) in human embryonic stem cells BJNhem20 using CRISPR-Cas9 gene targetingPratiksha P. Bhat0Maneesha S. Inamdar1Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, IndiaJawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India; Institute for Stem Cell Science and Regenerative Medicine (BRIC-inStem), Bangalore, India; Corresponding author at: Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India and Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, IndiaBeta actin is a cytoskeletal protein that contributes to a wide range of cellular processes. Here we generated beta actin reporter knock-in in BJNhem20 human embryonic stem cell line by CRISPR Cas9 gene editing. The reporter mEGFP is integrated at the beta actin locus, tagging the N-terminal of the protein via a linker. The reporter line is a valuable tool to study beta actin dynamics during cellular process in human embryonic stem cells and to track cells by live imaging.http://www.sciencedirect.com/science/article/pii/S1873506125001333 |
| spellingShingle | Pratiksha P. Bhat Maneesha S. Inamdar Generation of beta actin reporter line (BJNhem20 ACTB-eGFP) in human embryonic stem cells BJNhem20 using CRISPR-Cas9 gene targeting |
| title | Generation of beta actin reporter line (BJNhem20 ACTB-eGFP) in human embryonic stem cells BJNhem20 using CRISPR-Cas9 gene targeting |
| title_full | Generation of beta actin reporter line (BJNhem20 ACTB-eGFP) in human embryonic stem cells BJNhem20 using CRISPR-Cas9 gene targeting |
| title_fullStr | Generation of beta actin reporter line (BJNhem20 ACTB-eGFP) in human embryonic stem cells BJNhem20 using CRISPR-Cas9 gene targeting |
| title_full_unstemmed | Generation of beta actin reporter line (BJNhem20 ACTB-eGFP) in human embryonic stem cells BJNhem20 using CRISPR-Cas9 gene targeting |
| title_short | Generation of beta actin reporter line (BJNhem20 ACTB-eGFP) in human embryonic stem cells BJNhem20 using CRISPR-Cas9 gene targeting |
| title_sort | generation of beta actin reporter line bjnhem20 actb egfp in human embryonic stem cells bjnhem20 using crispr cas9 gene targeting |
| url | http://www.sciencedirect.com/science/article/pii/S1873506125001333 |
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