The nucleolin MoNsr1 plays pleiotropic roles in the pathogenicity and stress adaptation in the rice blast fungus Magnaporthe oryzae
The rice blast disease, caused by the fungus Magnaporthe oryzae, is a significant agricultural problem that adversely impacts rice production and food security. Understanding the precise molecular pathways involved in the interaction between the pathogen and its host is crucial for developing effect...
| Published in: | Frontiers in Plant Science |
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| Main Authors: | , , , , , , , , , , , |
| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-10-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1482934/full |
| _version_ | 1850353545567535104 |
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| author | Zhen Zhang Mohammad Shafiqul Islam Mohammad Shafiqul Islam Jiuzhi Xia Xiangyang Feng Muhammad Noman Jing Wang Zhongna Hao Haiping Qiu Rongyao Chai Yingying Cai Yanli Wang Jiaoyu Wang |
| author_facet | Zhen Zhang Mohammad Shafiqul Islam Mohammad Shafiqul Islam Jiuzhi Xia Xiangyang Feng Muhammad Noman Jing Wang Zhongna Hao Haiping Qiu Rongyao Chai Yingying Cai Yanli Wang Jiaoyu Wang |
| author_sort | Zhen Zhang |
| collection | DOAJ |
| container_title | Frontiers in Plant Science |
| description | The rice blast disease, caused by the fungus Magnaporthe oryzae, is a significant agricultural problem that adversely impacts rice production and food security. Understanding the precise molecular pathways involved in the interaction between the pathogen and its host is crucial for developing effective disease management strategies. This study examines the crucial function of the nucleolin MoNsr1 in regulating M. oryzae physiological functions. ΔMoNsr1 deletion mutants showed reduced fungal growth, asexual sporulation, and pathogenicity compared to the wild-type. Mutants exhibited impaired conidial germination and appressoria formation, reducing infection progression. Additionally, ΔMoNsr1 deletion mutant had less turgor pressure, confirming that MoNsr1 is essential for cell wall biogenesis and resistant to external stresses. Furthermore, ΔMoNsr1 deletion mutant showed enhanced sensitivity to oxidative stress, reactive oxygen species, and cold tolerance. Our results offer a thorough understanding of the function of MoNsr1 in the virulence and stress-resilient capability in M. oryzae. These findings provide insights into the novel targets and contribute to the emergence of innovative approaches for managing rice blast disease. |
| format | Article |
| id | doaj-art-a7a6a165b62b4e9da45b67de558be2be |
| institution | Directory of Open Access Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| spelling | doaj-art-a7a6a165b62b4e9da45b67de558be2be2025-08-19T23:08:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-10-011510.3389/fpls.2024.14829341482934The nucleolin MoNsr1 plays pleiotropic roles in the pathogenicity and stress adaptation in the rice blast fungus Magnaporthe oryzaeZhen Zhang0Mohammad Shafiqul Islam1Mohammad Shafiqul Islam2Jiuzhi Xia3Xiangyang Feng4Muhammad Noman5Jing Wang6Zhongna Hao7Haiping Qiu8Rongyao Chai9Yingying Cai10Yanli Wang11Jiaoyu Wang12State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory of Rice Biology and Breeding, Key Laboratory of Molecular Biology of Crop Pathogens and Insects of MARA, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaThe rice blast disease, caused by the fungus Magnaporthe oryzae, is a significant agricultural problem that adversely impacts rice production and food security. Understanding the precise molecular pathways involved in the interaction between the pathogen and its host is crucial for developing effective disease management strategies. This study examines the crucial function of the nucleolin MoNsr1 in regulating M. oryzae physiological functions. ΔMoNsr1 deletion mutants showed reduced fungal growth, asexual sporulation, and pathogenicity compared to the wild-type. Mutants exhibited impaired conidial germination and appressoria formation, reducing infection progression. Additionally, ΔMoNsr1 deletion mutant had less turgor pressure, confirming that MoNsr1 is essential for cell wall biogenesis and resistant to external stresses. Furthermore, ΔMoNsr1 deletion mutant showed enhanced sensitivity to oxidative stress, reactive oxygen species, and cold tolerance. Our results offer a thorough understanding of the function of MoNsr1 in the virulence and stress-resilient capability in M. oryzae. These findings provide insights into the novel targets and contribute to the emergence of innovative approaches for managing rice blast disease.https://www.frontiersin.org/articles/10.3389/fpls.2024.1482934/fullcell wall integrityMagnaporthe oryzaeMoNsr1nucleolinpathogenicitystress response |
| spellingShingle | Zhen Zhang Mohammad Shafiqul Islam Mohammad Shafiqul Islam Jiuzhi Xia Xiangyang Feng Muhammad Noman Jing Wang Zhongna Hao Haiping Qiu Rongyao Chai Yingying Cai Yanli Wang Jiaoyu Wang The nucleolin MoNsr1 plays pleiotropic roles in the pathogenicity and stress adaptation in the rice blast fungus Magnaporthe oryzae cell wall integrity Magnaporthe oryzae MoNsr1 nucleolin pathogenicity stress response |
| title | The nucleolin MoNsr1 plays pleiotropic roles in the pathogenicity and stress adaptation in the rice blast fungus Magnaporthe oryzae |
| title_full | The nucleolin MoNsr1 plays pleiotropic roles in the pathogenicity and stress adaptation in the rice blast fungus Magnaporthe oryzae |
| title_fullStr | The nucleolin MoNsr1 plays pleiotropic roles in the pathogenicity and stress adaptation in the rice blast fungus Magnaporthe oryzae |
| title_full_unstemmed | The nucleolin MoNsr1 plays pleiotropic roles in the pathogenicity and stress adaptation in the rice blast fungus Magnaporthe oryzae |
| title_short | The nucleolin MoNsr1 plays pleiotropic roles in the pathogenicity and stress adaptation in the rice blast fungus Magnaporthe oryzae |
| title_sort | nucleolin monsr1 plays pleiotropic roles in the pathogenicity and stress adaptation in the rice blast fungus magnaporthe oryzae |
| topic | cell wall integrity Magnaporthe oryzae MoNsr1 nucleolin pathogenicity stress response |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1482934/full |
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