Locking the GFP Fluorophore to Enhance Its Emission Intensity

The Green Fluorescent Protein (GFP) and its analogues have been widely used as fluorescent biomarkers in cell biology. Yet, the chromophore responsible for the fluorescence of the GFP is not emissive when isolated in solution, outside the protein environment. The most accepted explanation is that th...

Full description

Bibliographic Details
Published in:	Molecules
Main Authors:	Joana R. M. Ferreira, Cátia I. C. Esteves, Maria Manuel B. Marques, Samuel Guieu
Format:	Article
Language:	English
Published:	MDPI AG 2022-12-01
Subjects:	fluorescence green fluorescent protein Z/E isomerization aggregation-induced emission enhancement difluoroborate
Online Access:	https://www.mdpi.com/1420-3049/28/1/234

Similar Items

Multicomponent Synthesis of Luminescent Iminoboronates
by: Samuel Guieu, et al.
Published: (2020-12-01)

Anion-Complexation-Induced Emission Based on Aggregation-Induced Emission Fluorophore
by: Dongxing Ren, et al.
Published: (2023-02-01)

NIR-II and visible fluorescence hybrid imaging-guided surgery via aggregation-induced emission fluorophores cocktails
by: Xiaoxiao Fan, et al.
Published: (2022-12-01)

Tuning Photophysical Properties via Positional Isomerization of the Pyridine Ring in Donor–Acceptor-Structured Aggregation-Induced Emission Luminogens Based on Phenylmethylene Pyridineacetonitrile Derivatives
by: Haiya Sun, et al.
Published: (2023-04-01)

Co-Assembled Nanosystems Exhibiting Intrinsic Fluorescence by Complexation of Amino Terpolymer and Its Quaternized Analog with Aggregation-Induced Emission (AIE) Dye
by: Michaila Akathi Pantelaiou, et al.
Published: (2024-10-01)

Multicomponent Synthesis of New Fluorescent Boron Complexes Derived from 3-Hydroxy-1-phenyl-1<i>H</i>-pyrazole-4-carbaldehyde
by: Viktorija Savickienė, et al.
Published: (2024-07-01)

AIEgen configuration transition and aggregation enable dual prompt emission for single‐component nondoped white OLEDs
by: Jiasen Zhang, et al.
Published: (2024-02-01)

A simple “spraying” fluorescence‐guided surgery by AIE probes for liver tumor resection through configuration‐induced cross‐identification
by: Didi Chen, et al.
Published: (2024-08-01)

Tetraphenylethylene fluorophore-based AIE-fluorescent ionogels
by: Xuejing Zhai, et al.
Published: (2025-02-01)

Systematic Studies on the Effect of Fluorine Atoms in Fluorinated Tolanes on Their Photophysical Properties
by: Masato Morita, et al.
Published: (2021-04-01)

A Competitive “On-Off-Enhanced On” AIE Fluorescence Switch for Detecting Biothiols Based on Hg<sup>2+</sup> Ions and Gold Nanoclusters
by: Shuqi Li, et al.
Published: (2022-12-01)

Fluorogenic sensing of amorphous aggregates, amyloid fibers, and chaperone activity via a near‐infrared aggregation‐induced emission‐active probe
by: Wei He, et al.
Published: (2024-02-01)

Direct Detection and Quantification of Aqueous Proteins via a Fluorescent Probe Through the Use of Fluorophore-Induced Plasmonic Current
by: Daniel R. Pierce, et al.
Published: (2025-02-01)

Furan‐modified thiadiazolo quinoxaline as an electron acceptor for constructing second near‐infrared aggregation‐induced emission fluorophores for beyond 1300 nm fluorescence/photoacoustic imaging and photothermal therapy
by: Mengke Liang, et al.
Published: (2024-04-01)

Synthesis and Characterization of Diketopyrrolopyrrole-Based Aggregation-Induced Emission Nanoparticles for Bioimaging
by: Geonho Lee, et al.
Published: (2022-05-01)

Mesogens with aggregation‐induced emission properties: Materials with a bright future
by: Jens Voskuhl, et al.
Published: (2022-02-01)

Rapid induction of GFP expression by the nitrate reductase promoter in the diatom Phaeodactylum tricornutum
by: Lili Chu, et al.
Published: (2016-08-01)

Conjugated Aggregation-Induced Fluorescent Materials for Biofluorescent Probes: A Review
by: Zheng Wang, et al.
Published: (2023-01-01)

A Carbazole-Based Aggregation-Induced Emission “Turn-On” Sensor for Mercury Ions in Aqueous Solution
by: Remya Radha, et al.
Published: (2025-07-01)

Development of water-soluble AIE-based wash-free A probes superior to commercial ThT
by: Ting-Ting Hou, et al.
Published: (2023-11-01)

Developing Novel Fabrication and Optimisation Strategies on Aggregation-Induced Emission Nanoprobe/Polyvinyl Alcohol Hydrogels for Bio-Applications
by: Javad Tavakoli, et al.
Published: (2022-02-01)

An Innovative Aggregation-Induced Emission-Based NIR Fluorescent Probe for Visualizing Carboxylesterases in Living Cells, Zebrafish, and Tumor-Bearing Mice
by: Chao Gao, et al.
Published: (2024-08-01)

Confinement fluorescence effect of an aggregation‐induced emission luminogen in crystalline polymer
by: Lan Zhou, et al.
Published: (2023-10-01)

Fluorescent monitoring osteogenic differentiation of osteosarcoma cells with an aggregation-induced emission probe
by: Junxiong Zhang, et al.
Published: (2024-06-01)

Aggregation-Induced Emission (AIE) Probes in Fluorescent Sensing: Progress and Applications for Pesticide Detection
by: Bowen Zha, et al.
Published: (2024-10-01)

Molecular Rotors with Aggregation-Induced Emission (AIE) as Fluorescent Probes for the Control of Polyurethane Synthesis
by: Pierpaolo Minei, et al.
Published: (2020-12-01)

A specific aggregation-induced emission-conjugated polymer enables visual monitoring of osteogenic differentiation
by: Zhenyu Zheng, et al.
Published: (2020-12-01)

Methylsteric Effects Enhance Fluorescence in Diphenylfumaronitrile AIEgens
by: Zihao Xu, et al.
Published: (2025-07-01)

Synthesis and Application in Cell Imaging of Acridone Derivatives
by: Yung-Chieh Chan, et al.
Published: (2020-12-01)

A Phenanthroline-Based Fluorescent Probe for Highly Selective Detection of Extreme Alkalinity (pH > 14) in Aqueous Solution
by: Xiaoyu Ma, et al.
Published: (2019-09-01)

A visualizable strategy to real-time monitor chiral block copolypeptide assembly by AIE fluorescent probes
by: Zenghao Li, et al.
Published: (2024-12-01)

A Highly Efficient Fluorescent Sensor Based on AIEgen for Detection of Nitrophenolic Explosives
by: Dongmi Li, et al.
Published: (2022-12-01)

AIEgen-Peptide Bioprobes for the Imaging of Organelles
by: Bochao Chen, et al.
Published: (2022-08-01)

Photoelectric Multi-Signal Output Sensor Based on Two-Dimensional Covalent Organic Polymer Film Modified by Novel Aggregation-Induced Emission Probes
by: Yaru Song, et al.
Published: (2024-06-01)

Biosensors for the Detection of Enzymes Based on Aggregation-Induced Emission
by: Fengli Gao, et al.
Published: (2022-11-01)

Unveiling size‐fluorescence correlation of organic nanoparticles and its use in nanoparticle size determination
by: Wu‐Jie Guo, et al.
Published: (2024-02-01)

C─H···π interaction induced H‐aggregates for wide range water content detection in organic solvents
by: Jiajun Xu, et al.
Published: (2024-08-01)

Machine-learning screening of luminogens with aggregation-induced emission characteristics for fluorescence imaging
by: Yibin Zhang, et al.
Published: (2023-03-01)

A Novel Aggregation-Induced Emission Fluorescent Probe for Detection of β-Amyloid Based on Pyridinyltriphenylamine and Quinoline–Malononitrile
by: Yan Fang, et al.
Published: (2023-06-01)

Multi‐state photoluminescent properties of an overcrowded alkene‐based molecular motor in aggregates
by: Yahan Shan, et al.
Published: (2024-10-01)