| 要約: | The study was to optimize the extraction process of flavonoids from of <italic>Hedychium flavum</italic> Roxb stem. Ultrasonic-assisted ethanol extraction was used to extract flavonoids from <italic>Hedychium flavum</italic> Roxb stem. Based on the single factor experiment, the extraction amount of flavonoids was used as the response value. Box-Behnken design and optimization were carried out on the four factors affecting the extraction amount of flavonoids from <italic>Hedychium flavum</italic> Roxb stem, analyzed the effect of ethanol concentration, liquid to solid ratio, temperature and extraction time on the extraction amount of flavonoids from <italic>Hedychium flavum</italic> Roxb stem, determined the <italic>in vitro</italic> antioxidant activity of flavonoid extracts from <italic>Hedychium flavum</italic> Roxb stem. The results showed that the optimum extraction conditions were liquid to solid of 15 mL/g, the temperature of 63.0 ℃, the extraction time of 32.5 min, and the ethanol concentration of 90%. Under the optimum conditions, the extraction amount of flavonoids from <italic>Hedychium flavum</italic> Roxb stem was 47.97 mg/g, which was basically consistent with the predicted value, indicating that the optimization method was reasonable and reliable. The <italic>in vitro</italic> antioxidant test showed that the flavonoid extract of the rhizome had strong DPPH and ABTS scavenging ability. The study indicates that the flavonoid extract of the rhizome had good antioxidant activity.
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