Multiplexed Spectral Imaging of 120 Different Fluorescent Labels.

The number of fluorescent labels that can unambiguously be distinguished in a single image when acquired through band pass filters is severely limited by the spectral overlap of available fluorophores. The recent development of spectral microscopy and the application of linear unmixing algorithms to...

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Bibliographic Details
Published in:PLoS ONE
Main Authors: Alex M Valm, Rudolf Oldenbourg, Gary G Borisy
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Online Access:http://europepmc.org/articles/PMC4938436?pdf=render

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