| 總結: | Background: Drought stress is a major abiotic factor limiting <i>Brassica juncea</i> productivity, resulting in significant yield reductions. Plant Growth-Promoting Rhizobacteria (PGPR) have shown potential in enhancing drought tolerance; however, the metabolomic changes associated with their effects remain largely unexplored. This study examines the metabolic changes induced by a PGPR consortium (<i>Enterobacter hormaechei</i>, <i>Pantoea dispersa</i>, and <i>Acinetobacter</i> sp.) in two contrasting genotypes <i>B. juncea</i> (L.) Czern. ‘RH 725’ (drought tolerant) and <i>B. juncea</i> (L.) Czern. ‘RH-749’ (drought sensitive for drought tolerance, under both control and drought conditions. Methods: Metabolite profiling was conducted using gas chromatography-mass spectrometry (GC-MS) to identify compounds that accumulated differentially across treatments. We applied multivariate statistical methods, such as Partial Least Squares Discriminant Analysis (PLS-DA), hierarchical clustering, and pathway enrichment analysis, to explore metabolic reprogramming. Results: Drought stress induced significant changes in metabolite profile, particularly increasing the levels of osmoprotectants such as trehalose, glucose, sucrose, proline, and valine. Additionally, alterations in organic acids (malic acid and citric acid) and fatty acids (oleic acid and linoleic acid) were observed. PGPR inoculation further amplified these metabolic responses to enhance the osmotic regulation, reactive oxygen species (ROS) detoxification, and carbon-nitrogen metabolism, with RH-725 displaying a stronger adaptive response. Pathway enrichment analysis revealed that PGPR treatment significantly influenced metabolic pathways related to starch and sucrose metabolism, galactose metabolism, and amino acid biosynthesis, which play critical roles in drought adaptation. Conclusion: These findings provide insights into how PGPR contributes to stress resilience in <i>B. juncea</i> by modulating key biochemical pathways. This study provides new molecular insights into the known effect of PGPR for mitigating drought stress in oilseed crops.
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