Multibioreaction Methodology for Baeyer-Villiger Monooxygenase Monitoring

• Published in: Food Technology and Biotechnology
• Main Authors: Regina A. C. Gonçalves, André L. M. Porto, Lucimar Pinheiro, José R. Cagnon, Gilson P. Manfio, Anita J. Marsaioli
• Format: Article
• Language: English
• Published: University of Zagreb Faculty of Food Technology and Biotechnology 2004-01-01
• Subjects: Baeyer-Villiger monooxygenase; alkene monooxygenase; multibioreaction
• Online Access: http://hrcak.srce.hr/file/163432
• ISSN: 1330-9862; 1334-2606

Baeyer-Villiger monooxygenase (BVMO) activity was monitored using traditional biocatalytic methods and also using a multibioreaction approach. The prochiral ketones 4-methyl- cyclohexanone and 3-hexyl-cyclobutanone, among others, were used in screening for BVMO in several microorganisms, leading to the selection of Geotrichum candidum CCT 1205, Aspergillus oryzae CCT 0975, Curvularia lunata CCT 5629, Aspergillus niger CCT 5559, Trichoderma sp. CCT 5551, Cunninghamella echinulata CCT 4424 and Cunninghamella echinulata CCT 4259 as good candidates for further BVMO investigations. Additionally, a multibioreaction methodology was used to confirm the presence of BVMO, an activity previously detected by a rapid fluorescence methodology. It was therefore possible to confirm the presence of a BVMO, more precisely a cyclohexanone monooxygenase (CHMO) and also to reveal the presence of an alkene monooxygenase in Trichosporum cutaneum CCT 1903.