Production and application of monoclonal antibodies to GST
Two hybridoma cell lines (4B3 and 1H10) secreting monoclonal antibodies (McAbs) against recombinant GST were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/C immunized by the purified GST protein and subsequently three-time limited dilution method. The titres of ascitic f...
| Published in: | 浙江大学学报. 农业与生命科学版 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2006-07-01
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| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2006.04.0383 |
| Summary: | Two hybridoma cell lines (4B3 and 1H10) secreting monoclonal antibodies (McAbs) against recombinant GST were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/C immunized by the purified GST protein and subsequently three-time limited dilution method. The titres of ascitic fluids of the two McAbs were up to 10<sup>-6</sup> in indirect ELISA. Isotypes and subclasses of 1H10 and 4B3 all belong to IgG1, κ. Western-blot showed that the two McAbs can specifically react with GST-IBV-S1-F and recombinant GST. The McAb 1H10 has successfully been used in immunoaffinity chromatography of GST fusion protein. |
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| ISSN: | 1008-9209 2097-5155 |