Higher Phytohormone Contents and Weaker Phytohormone Signal Transduction Were Observed in Cold-Tolerant Cucumber
Cucumbers (<i>Cucumis sativus</i> L.) originated from the South Asian subcontinent, and most of them are fragile to cold stress. In this study, we evaluated the cold tolerance of 115 cucumber accessions and screened out 10 accessions showing high resistance to cold stress. We measured an...
| 发表在: | Plants |
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| Main Authors: | , , , , , , , , , |
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| 语言: | 英语 |
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MDPI AG
2022-04-01
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| 在线阅读: | https://www.mdpi.com/2223-7747/11/7/961 |
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| author | Radwa Salah Rui-Jin Zhang Shi-Wei Xia Shan-Shan Song Qian Hao Mustafa H. Hashem Huan-Xiu Li Yu Li Xi-Xiang Li Yun-Song Lai |
| author_facet | Radwa Salah Rui-Jin Zhang Shi-Wei Xia Shan-Shan Song Qian Hao Mustafa H. Hashem Huan-Xiu Li Yu Li Xi-Xiang Li Yun-Song Lai |
| author_sort | Radwa Salah |
| collection | DOAJ |
| container_title | Plants |
| description | Cucumbers (<i>Cucumis sativus</i> L.) originated from the South Asian subcontinent, and most of them are fragile to cold stress. In this study, we evaluated the cold tolerance of 115 cucumber accessions and screened out 10 accessions showing high resistance to cold stress. We measured and compared plant hormone contents between cold-tolerant cucumber CT90R and cold-sensitive cucumber CT57S in cold treatment. Most of the detected plant hormones showed significantly higher content in CT90R. To elucidate the role of plant hormones, we compared the leaf- and root-transcriptomes of CT90R with those of CT57S in cold stress treatment. In leaves, there were 1209 differentially expressed genes (DEGs) between CT90R and CT57S, while there were 703 in roots. These DEGs were not evenly distributed across the chromosomes and there were significant enrichments at particular positions, including <i>qLTT6.2</i>, a known QTL controlling cucumber cold tolerance. The GO and KEGG enrichment analysis showed that there was a significant difference in the pathway of plant hormone transductions between CT90R and CT57S in leaves. In short, genes involved in plant hormone transductions showed lower transcription levels in CT90R. In roots, the most significantly different pathway was phenylpropanoid biosynthesis. CT90R seemed to actively accumulate more monolignols by upregulating <i>cinnamyl-alcohol</i> <i>dehydrogenase</i> (<i>CAD</i>) genes. These results above suggest a new perspective on the regulation mechanism of cold tolerance in cucumbers. |
| format | Article |
| id | doaj-art-bcbb69ad3a3a4daeb11ddff0d4572d7b |
| institution | Directory of Open Access Journals |
| issn | 2223-7747 |
| language | English |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-bcbb69ad3a3a4daeb11ddff0d4572d7b2025-08-19T23:53:12ZengMDPI AGPlants2223-77472022-04-0111796110.3390/plants11070961Higher Phytohormone Contents and Weaker Phytohormone Signal Transduction Were Observed in Cold-Tolerant CucumberRadwa Salah0Rui-Jin Zhang1Shi-Wei Xia2Shan-Shan Song3Qian Hao4Mustafa H. Hashem5Huan-Xiu Li6Yu Li7Xi-Xiang Li8Yun-Song Lai9College of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaInstitution of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, South Zhongguancun Street 12, Beijing 100081, ChinaCollege of Horticulture, Sichuan Agricultural University, Chengdu 611130, ChinaCucumbers (<i>Cucumis sativus</i> L.) originated from the South Asian subcontinent, and most of them are fragile to cold stress. In this study, we evaluated the cold tolerance of 115 cucumber accessions and screened out 10 accessions showing high resistance to cold stress. We measured and compared plant hormone contents between cold-tolerant cucumber CT90R and cold-sensitive cucumber CT57S in cold treatment. Most of the detected plant hormones showed significantly higher content in CT90R. To elucidate the role of plant hormones, we compared the leaf- and root-transcriptomes of CT90R with those of CT57S in cold stress treatment. In leaves, there were 1209 differentially expressed genes (DEGs) between CT90R and CT57S, while there were 703 in roots. These DEGs were not evenly distributed across the chromosomes and there were significant enrichments at particular positions, including <i>qLTT6.2</i>, a known QTL controlling cucumber cold tolerance. The GO and KEGG enrichment analysis showed that there was a significant difference in the pathway of plant hormone transductions between CT90R and CT57S in leaves. In short, genes involved in plant hormone transductions showed lower transcription levels in CT90R. In roots, the most significantly different pathway was phenylpropanoid biosynthesis. CT90R seemed to actively accumulate more monolignols by upregulating <i>cinnamyl-alcohol</i> <i>dehydrogenase</i> (<i>CAD</i>) genes. These results above suggest a new perspective on the regulation mechanism of cold tolerance in cucumbers.https://www.mdpi.com/2223-7747/11/7/961<i>Cucumis sativus</i>RNA-seqtranscriptomecold toleranceplant hormone |
| spellingShingle | Radwa Salah Rui-Jin Zhang Shi-Wei Xia Shan-Shan Song Qian Hao Mustafa H. Hashem Huan-Xiu Li Yu Li Xi-Xiang Li Yun-Song Lai Higher Phytohormone Contents and Weaker Phytohormone Signal Transduction Were Observed in Cold-Tolerant Cucumber <i>Cucumis sativus</i> RNA-seq transcriptome cold tolerance plant hormone |
| title | Higher Phytohormone Contents and Weaker Phytohormone Signal Transduction Were Observed in Cold-Tolerant Cucumber |
| title_full | Higher Phytohormone Contents and Weaker Phytohormone Signal Transduction Were Observed in Cold-Tolerant Cucumber |
| title_fullStr | Higher Phytohormone Contents and Weaker Phytohormone Signal Transduction Were Observed in Cold-Tolerant Cucumber |
| title_full_unstemmed | Higher Phytohormone Contents and Weaker Phytohormone Signal Transduction Were Observed in Cold-Tolerant Cucumber |
| title_short | Higher Phytohormone Contents and Weaker Phytohormone Signal Transduction Were Observed in Cold-Tolerant Cucumber |
| title_sort | higher phytohormone contents and weaker phytohormone signal transduction were observed in cold tolerant cucumber |
| topic | <i>Cucumis sativus</i> RNA-seq transcriptome cold tolerance plant hormone |
| url | https://www.mdpi.com/2223-7747/11/7/961 |
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