Stem-Cell-Regenerative and Protective Effects of Squid (<i>Symplectoteuthis oualaniensis</i>) Skin Collagen Peptides against H<sub>2</sub>O<sub>2</sub>-Induced Fibroblast Injury
Recently, there has been a growing interest in collagen peptides derived from marine sources for their notable ability to protect skin cells against apoptosis induced by oxidants. Therefore, the current study aimed to investigate the fundamental properties of collagen peptides, including their physi...
| Published in: | Marine Drugs |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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MDPI AG
2024-05-01
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| Online Access: | https://www.mdpi.com/1660-3397/22/6/255 |
| _version_ | 1850279890506481664 |
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| author | Mingjun Wei Lakshmi Jeevithan Na Li Lixin Liu Jiren Xu Wenhui Wu Jeevithan Elango |
| author_facet | Mingjun Wei Lakshmi Jeevithan Na Li Lixin Liu Jiren Xu Wenhui Wu Jeevithan Elango |
| author_sort | Mingjun Wei |
| collection | DOAJ |
| container_title | Marine Drugs |
| description | Recently, there has been a growing interest in collagen peptides derived from marine sources for their notable ability to protect skin cells against apoptosis induced by oxidants. Therefore, the current study aimed to investigate the fundamental properties of collagen peptides, including their physicochemical, thermal, structural, stem-cell-regenerative, and skin-cell-protective effects, in comparison to commercial collagen peptides. The acid-soluble (ASC) and pepsin-soluble (PSC) collagens exhibited three distinct bands on SDS-PAGE, namely α (α<sub>1</sub> and α<sub>2</sub>), β, and γ chains, confirming a type I pattern. The thermal profiles obtained from TG and DSC analyses confirmed the denaturation of PSC and ASC at temperatures ranging from 51.94 to 56.4 °C and from 52.07 to 56.53 °C, respectively. The purified collagen peptides were analyzed using SDS-PAGE and MALDI-TOF mass spectrometry, revealing a mass range of 900–15,000 Da. Furthermore, the de novo peptide sequence analysis confirmed the presence of the Gly-X-Y repeating sequence in collagen peptides. Collagen peptide treatments significantly enhanced HFF-1 cell proliferation and migration compared to the control group. ELISA results confirmed the potential interactions between collagen peptides and HFF-1 cells through α<sub>2</sub>β<sub>1</sub>, α<sub>10</sub>β<sub>1</sub>, and α<sub>11</sub>β<sub>1</sub> integrin receptors. Notably, collagen peptide treatment effectively restored the proliferation of HFF-1 cells damaged by H<sub>2</sub>O<sub>2</sub>. Consequently, the advantageous characteristics of squid skin collagen peptides highlight their promising role in regenerative medicine. |
| format | Article |
| id | doaj-art-bcbdcbfe01424d6c96e225dad5ece4fe |
| institution | Directory of Open Access Journals |
| issn | 1660-3397 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-bcbdcbfe01424d6c96e225dad5ece4fe2025-08-19T23:39:38ZengMDPI AGMarine Drugs1660-33972024-05-0122625510.3390/md22060255Stem-Cell-Regenerative and Protective Effects of Squid (<i>Symplectoteuthis oualaniensis</i>) Skin Collagen Peptides against H<sub>2</sub>O<sub>2</sub>-Induced Fibroblast InjuryMingjun Wei0Lakshmi Jeevithan1Na Li2Lixin Liu3Jiren Xu4Wenhui Wu5Jeevithan Elango6Department of Marine Biopharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaDepartment of Marine Biopharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaDepartment of Marine Biopharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaDepartment of Marine Biopharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaDepartment of Marine Biopharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaDepartment of Marine Biopharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaDepartment of Marine Biopharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaRecently, there has been a growing interest in collagen peptides derived from marine sources for their notable ability to protect skin cells against apoptosis induced by oxidants. Therefore, the current study aimed to investigate the fundamental properties of collagen peptides, including their physicochemical, thermal, structural, stem-cell-regenerative, and skin-cell-protective effects, in comparison to commercial collagen peptides. The acid-soluble (ASC) and pepsin-soluble (PSC) collagens exhibited three distinct bands on SDS-PAGE, namely α (α<sub>1</sub> and α<sub>2</sub>), β, and γ chains, confirming a type I pattern. The thermal profiles obtained from TG and DSC analyses confirmed the denaturation of PSC and ASC at temperatures ranging from 51.94 to 56.4 °C and from 52.07 to 56.53 °C, respectively. The purified collagen peptides were analyzed using SDS-PAGE and MALDI-TOF mass spectrometry, revealing a mass range of 900–15,000 Da. Furthermore, the de novo peptide sequence analysis confirmed the presence of the Gly-X-Y repeating sequence in collagen peptides. Collagen peptide treatments significantly enhanced HFF-1 cell proliferation and migration compared to the control group. ELISA results confirmed the potential interactions between collagen peptides and HFF-1 cells through α<sub>2</sub>β<sub>1</sub>, α<sub>10</sub>β<sub>1</sub>, and α<sub>11</sub>β<sub>1</sub> integrin receptors. Notably, collagen peptide treatment effectively restored the proliferation of HFF-1 cells damaged by H<sub>2</sub>O<sub>2</sub>. Consequently, the advantageous characteristics of squid skin collagen peptides highlight their promising role in regenerative medicine.https://www.mdpi.com/1660-3397/22/6/255squid collagen peptidesskin cellsH<sub>2</sub>O<sub>2</sub> oxidative damageregenerative medicine |
| spellingShingle | Mingjun Wei Lakshmi Jeevithan Na Li Lixin Liu Jiren Xu Wenhui Wu Jeevithan Elango Stem-Cell-Regenerative and Protective Effects of Squid (<i>Symplectoteuthis oualaniensis</i>) Skin Collagen Peptides against H<sub>2</sub>O<sub>2</sub>-Induced Fibroblast Injury squid collagen peptides skin cells H<sub>2</sub>O<sub>2</sub> oxidative damage regenerative medicine |
| title | Stem-Cell-Regenerative and Protective Effects of Squid (<i>Symplectoteuthis oualaniensis</i>) Skin Collagen Peptides against H<sub>2</sub>O<sub>2</sub>-Induced Fibroblast Injury |
| title_full | Stem-Cell-Regenerative and Protective Effects of Squid (<i>Symplectoteuthis oualaniensis</i>) Skin Collagen Peptides against H<sub>2</sub>O<sub>2</sub>-Induced Fibroblast Injury |
| title_fullStr | Stem-Cell-Regenerative and Protective Effects of Squid (<i>Symplectoteuthis oualaniensis</i>) Skin Collagen Peptides against H<sub>2</sub>O<sub>2</sub>-Induced Fibroblast Injury |
| title_full_unstemmed | Stem-Cell-Regenerative and Protective Effects of Squid (<i>Symplectoteuthis oualaniensis</i>) Skin Collagen Peptides against H<sub>2</sub>O<sub>2</sub>-Induced Fibroblast Injury |
| title_short | Stem-Cell-Regenerative and Protective Effects of Squid (<i>Symplectoteuthis oualaniensis</i>) Skin Collagen Peptides against H<sub>2</sub>O<sub>2</sub>-Induced Fibroblast Injury |
| title_sort | stem cell regenerative and protective effects of squid i symplectoteuthis oualaniensis i skin collagen peptides against h sub 2 sub o sub 2 sub induced fibroblast injury |
| topic | squid collagen peptides skin cells H<sub>2</sub>O<sub>2</sub> oxidative damage regenerative medicine |
| url | https://www.mdpi.com/1660-3397/22/6/255 |
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