Protocol for neonatal respiratory syncytial virus infection in mice and immune analysis of infected lungs and bronchoalveolar lavage fluid

• 出版年: STAR Protocols
• 主要な著者: Sandeep Adhikari, Pankaj Baral
• フォーマット: 論文
• 言語: 英語
• 出版事項: Elsevier 2023-09-01
• 主題: Flow Cytometry/Mass Cytometry; Immunology; Microbiology; Model Organisms
• オンライン･アクセス: http://www.sciencedirect.com/science/article/pii/S266616672300401X
• ISSN: 2666-1667

Summary: Respiratory syncytial virus (RSV) infection in infants and toddlers is a major public health problem. Here, we provide a protocol for neonatal RSV infection in mice and immune analysis of infected lungs and bronchoalveolar lavage (BAL) fluid. We describe steps for anesthesia and intranasal inoculation, weight monitoring, and whole lung collection. We then detail BAL fluid immune and whole lung analyses. This protocol can be used for neonatal pulmonary infection with other viruses or bacteria. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.