| Summary: | We describe the complete mitochondrial genome of the broadclub cuttlefish Sepia latimanus, the second largest cuttlefish, collected from the coral waters of Naozhou Island in tropical South China. It is a circular molecule of 16,225 bp in size and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 control region in typical gene arrangement conforming to the vertebrate consensus. The complete mitochondrial genomic sequence of S. latimanus and other 8 species from the same Family Sepiidae were used for phylogenetic analysis and demonstrated that S. latimanus clustered with S. apama, the largest cuttlefish species, and made a separate clade, closely related to the other Sepia and Sepiella species in Family Sepiidae.
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