Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes
Summary: Cryopreservation has a negative effect on the quality of oocytes and may be closely associated with increased levels of reactive oxygen species (ROS) and apoptotic events. The purpose of the present study was to evaluate the detrimental effects on the developmental competence of somatic cel...
| Published in: | Stem Cell Reports |
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| Main Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | English |
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Elsevier
2019-03-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671119300219 |
| _version_ | 1849316495466692608 |
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| author | Ah Reum Lee Kwonho Hong Seo Hye Choi Chanhyeok Park Jae Kyun Park Jin Il Lee Jae Il Bang Dong-Won Seol Jeoung Eun Lee Dong Ryul Lee |
| author_facet | Ah Reum Lee Kwonho Hong Seo Hye Choi Chanhyeok Park Jae Kyun Park Jin Il Lee Jae Il Bang Dong-Won Seol Jeoung Eun Lee Dong Ryul Lee |
| author_sort | Ah Reum Lee |
| collection | DOAJ |
| container_title | Stem Cell Reports |
| description | Summary: Cryopreservation has a negative effect on the quality of oocytes and may be closely associated with increased levels of reactive oxygen species (ROS) and apoptotic events. The purpose of the present study was to evaluate the detrimental effects on the developmental competence of somatic cell nuclear transferred (SCNT) mouse embryos using vitrified (cryopreserved) oocytes and to evaluate the recovery effects of melatonin on cryo-damage in cloned embryos. Development of SCNT embryos using cryopreserved oocyte cytoplasm (SCNT-CROC) was inferior to those using fresh cytoplasm (SCNT-FOC). Using RNA-sequencing analysis, we found upregulation of eight pro-apoptotic-related genes (Cyct, Dapk2, Dffb, Gadd45g, Hint2, Mien1, P2rx7, and Pmaip) in the SCNT-CROC group. Furthermore, the addition of melatonin, an agent that reduces apoptosis and ROS production, enhanced blastocyst formation rates in the SCNT-CROP group when compared with the melatonin-untreated group. Additionally, melatonin treatment increased the derivation efficiency of pluripotent stem cells from cloned embryos using cryopreserved oocyte. : In this article, Lee and colleagues found that the low developmental efficiency of cloned mouse embryos using cryopreserved oocyte cytoplasm may be due to increased apoptosis and altered gene expression resulting from cryoinjury. Therefore, supplementation of melatonin, a scavenger agent, may positively affect the quality of cloned embryos by regulating gene expression and apoptotic processes. Keywords: melatonin, cryopreserved oocytes, somatic cell nuclear transfer, embryonic development, cryo-damage |
| format | Article |
| id | doaj-art-c165e111b8d84df9803702f08ef50abe |
| institution | Directory of Open Access Journals |
| issn | 2213-6711 |
| language | English |
| publishDate | 2019-03-01 |
| publisher | Elsevier |
| record_format | Article |
| spelling | doaj-art-c165e111b8d84df9803702f08ef50abe2025-09-02T22:45:59ZengElsevierStem Cell Reports2213-67112019-03-0112354555610.1016/j.stemcr.2019.01.019Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved OocytesAh Reum Lee0Kwonho Hong1Seo Hye Choi2Chanhyeok Park3Jae Kyun Park4Jin Il Lee5Jae Il Bang6Dong-Won Seol7Jeoung Eun Lee8Dong Ryul Lee9Department of Biomedical Science, College of Life Science, CHA University, 335 Pankyo-ro, Seongnam-si, Gyeonggi-do, 13488 Seoul, KoreaDepartment of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul, KoreaDepartment of Biomedical Science, College of Life Science, CHA University, 335 Pankyo-ro, Seongnam-si, Gyeonggi-do, 13488 Seoul, KoreaDepartment of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul, KoreaFertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, KoreaFertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, KoreaDepartment of Biomedical Science, College of Life Science, CHA University, 335 Pankyo-ro, Seongnam-si, Gyeonggi-do, 13488 Seoul, KoreaDepartment of Biomedical Science, College of Life Science, CHA University, 335 Pankyo-ro, Seongnam-si, Gyeonggi-do, 13488 Seoul, KoreaCHA Stem Cell Institute, CHA University, Seongnam, KoreaDepartment of Biomedical Science, College of Life Science, CHA University, 335 Pankyo-ro, Seongnam-si, Gyeonggi-do, 13488 Seoul, Korea; CHA Stem Cell Institute, CHA University, Seongnam, Korea; Corresponding authorSummary: Cryopreservation has a negative effect on the quality of oocytes and may be closely associated with increased levels of reactive oxygen species (ROS) and apoptotic events. The purpose of the present study was to evaluate the detrimental effects on the developmental competence of somatic cell nuclear transferred (SCNT) mouse embryos using vitrified (cryopreserved) oocytes and to evaluate the recovery effects of melatonin on cryo-damage in cloned embryos. Development of SCNT embryos using cryopreserved oocyte cytoplasm (SCNT-CROC) was inferior to those using fresh cytoplasm (SCNT-FOC). Using RNA-sequencing analysis, we found upregulation of eight pro-apoptotic-related genes (Cyct, Dapk2, Dffb, Gadd45g, Hint2, Mien1, P2rx7, and Pmaip) in the SCNT-CROC group. Furthermore, the addition of melatonin, an agent that reduces apoptosis and ROS production, enhanced blastocyst formation rates in the SCNT-CROP group when compared with the melatonin-untreated group. Additionally, melatonin treatment increased the derivation efficiency of pluripotent stem cells from cloned embryos using cryopreserved oocyte. : In this article, Lee and colleagues found that the low developmental efficiency of cloned mouse embryos using cryopreserved oocyte cytoplasm may be due to increased apoptosis and altered gene expression resulting from cryoinjury. Therefore, supplementation of melatonin, a scavenger agent, may positively affect the quality of cloned embryos by regulating gene expression and apoptotic processes. Keywords: melatonin, cryopreserved oocytes, somatic cell nuclear transfer, embryonic development, cryo-damagehttp://www.sciencedirect.com/science/article/pii/S2213671119300219 |
| spellingShingle | Ah Reum Lee Kwonho Hong Seo Hye Choi Chanhyeok Park Jae Kyun Park Jin Il Lee Jae Il Bang Dong-Won Seol Jeoung Eun Lee Dong Ryul Lee Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes |
| title | Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes |
| title_full | Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes |
| title_fullStr | Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes |
| title_full_unstemmed | Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes |
| title_short | Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes |
| title_sort | anti apoptotic regulation contributes to the successful nuclear reprogramming using cryopreserved oocytes |
| url | http://www.sciencedirect.com/science/article/pii/S2213671119300219 |
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