Microsatellite Marker for Cross-Species Amplification: Study Case for Indonesian Sundaland Python (Serpentes: Pythonidae)
The python of Indonesian Sundaland has been traded for its distinct skin colour and patterns. The need for rapid method in cross-species amplification for Indonesian Sundaland python is useful to contribute in management of sustainability harvesting system. In this research, we screened 10 microsate...
| الحاوية / القاعدة: | Journal of Experimental Life Science |
|---|---|
| المؤلفون الرئيسيون: | , , , |
| التنسيق: | مقال |
| اللغة: | الإنجليزية |
| منشور في: |
University of Brawijaya
2018-02-01
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://jels.ub.ac.id/index.php/jels/article/view/252 |
| _version_ | 1849716321829257216 |
|---|---|
| author | Andri Maulidi Fatchiyah Fatchiyah Amir Hamidy Nia Kurniawan |
| author_facet | Andri Maulidi Fatchiyah Fatchiyah Amir Hamidy Nia Kurniawan |
| author_sort | Andri Maulidi |
| collection | DOAJ |
| container_title | Journal of Experimental Life Science |
| description | The python of Indonesian Sundaland has been traded for its distinct skin colour and patterns. The need for rapid method in cross-species amplification for Indonesian Sundaland python is useful to contribute in management of sustainability harvesting system. In this research, we screened 10 microsatellite primers which are previously used for Australian, New Guinean, Chinese and Burmese pythons and 7 potentially amplifiable primers for African and Asian reptiles. Python breitensteini showed a greater number of alleles (2-8 alleles) than Python bivittatus (1-3 alleles) and Python brongersmai (1-2 alleles). The observed and expected heterozygosity for all species were ranged from 0 to 1.00 and 0 to 0.79, respectively. According to the high cross-species amplification rates, 15 out of 17 primers were useful in assessing the genetic diversity and conservation genetic of Indonesian Sundaland python. Among the 15 primers, MS3 generated the highest number of allele for P. breitensteini (8 alleles), P. bivittatus (3 alleles), and P. brongersmai (2 alleles). We proposed MS3 locus as a suitable marker for Indonesian Sundaland python. |
| format | Article |
| id | doaj-art-c2b97ffaf73649f39cb74e0ef905cd2b |
| institution | Directory of Open Access Journals |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2018-02-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| spelling | doaj-art-c2b97ffaf73649f39cb74e0ef905cd2b2025-08-20T01:54:57ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552018-02-01816165https://doi.org/10.21776/ub.jels.2018.008.01.10Microsatellite Marker for Cross-Species Amplification: Study Case for Indonesian Sundaland Python (Serpentes: Pythonidae)Andri Maulidi0Fatchiyah Fatchiyah1Amir Hamidy2Nia Kurniawan3Universitas BrawijayaUniversitas BrawijayaLaboratory of Herpetology, Museum Zoologicum Bogoriense, Research Center for Biology, Indonesian Institute of Sciences LIPIUniversitas BrawijayaThe python of Indonesian Sundaland has been traded for its distinct skin colour and patterns. The need for rapid method in cross-species amplification for Indonesian Sundaland python is useful to contribute in management of sustainability harvesting system. In this research, we screened 10 microsatellite primers which are previously used for Australian, New Guinean, Chinese and Burmese pythons and 7 potentially amplifiable primers for African and Asian reptiles. Python breitensteini showed a greater number of alleles (2-8 alleles) than Python bivittatus (1-3 alleles) and Python brongersmai (1-2 alleles). The observed and expected heterozygosity for all species were ranged from 0 to 1.00 and 0 to 0.79, respectively. According to the high cross-species amplification rates, 15 out of 17 primers were useful in assessing the genetic diversity and conservation genetic of Indonesian Sundaland python. Among the 15 primers, MS3 generated the highest number of allele for P. breitensteini (8 alleles), P. bivittatus (3 alleles), and P. brongersmai (2 alleles). We proposed MS3 locus as a suitable marker for Indonesian Sundaland python.https://jels.ub.ac.id/index.php/jels/article/view/252microsatellitepythonsundaland |
| spellingShingle | Andri Maulidi Fatchiyah Fatchiyah Amir Hamidy Nia Kurniawan Microsatellite Marker for Cross-Species Amplification: Study Case for Indonesian Sundaland Python (Serpentes: Pythonidae) microsatellite python sundaland |
| title | Microsatellite Marker for Cross-Species Amplification: Study Case for Indonesian Sundaland Python (Serpentes: Pythonidae) |
| title_full | Microsatellite Marker for Cross-Species Amplification: Study Case for Indonesian Sundaland Python (Serpentes: Pythonidae) |
| title_fullStr | Microsatellite Marker for Cross-Species Amplification: Study Case for Indonesian Sundaland Python (Serpentes: Pythonidae) |
| title_full_unstemmed | Microsatellite Marker for Cross-Species Amplification: Study Case for Indonesian Sundaland Python (Serpentes: Pythonidae) |
| title_short | Microsatellite Marker for Cross-Species Amplification: Study Case for Indonesian Sundaland Python (Serpentes: Pythonidae) |
| title_sort | microsatellite marker for cross species amplification study case for indonesian sundaland python serpentes pythonidae |
| topic | microsatellite python sundaland |
| url | https://jels.ub.ac.id/index.php/jels/article/view/252 |
| work_keys_str_mv | AT andrimaulidi microsatellitemarkerforcrossspeciesamplificationstudycaseforindonesiansundalandpythonserpentespythonidae AT fatchiyahfatchiyah microsatellitemarkerforcrossspeciesamplificationstudycaseforindonesiansundalandpythonserpentespythonidae AT amirhamidy microsatellitemarkerforcrossspeciesamplificationstudycaseforindonesiansundalandpythonserpentespythonidae AT niakurniawan microsatellitemarkerforcrossspeciesamplificationstudycaseforindonesiansundalandpythonserpentespythonidae |