Disperse Orange 3 as a resonance Raman probe for measuring membrane order

Resonance Raman spectra of azobenzene derivatives were examined in the presence of lipid membranes to find a probe that can distinguish different membrane phases. The NO2 symmetric stretching band of 4‐(4‐nitrophenylazo)aniline, also known as Disperse Orange 3 (DO3), is downshifted by about 4 cm−1 o...

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Bibliographic Details
Published in:FEBS Open Bio
Main Authors: Yuki Numakura, Takashi Miura
Format: Article
Language:English
Published: Wiley 2015-01-01
Subjects:
Membrane order
Disperse Orange 3
Raman spectroscopy
Resonance Raman probe
Membrane microdomains
Online Access:https://doi.org/10.1016/j.fob.2015.10.008
Description
Summary:Resonance Raman spectra of azobenzene derivatives were examined in the presence of lipid membranes to find a probe that can distinguish different membrane phases. The NO2 symmetric stretching band of 4‐(4‐nitrophenylazo)aniline, also known as Disperse Orange 3 (DO3), is downshifted by about 4 cm−1 on the phase transition of phosphatidylcholine membranes from the liquid crystalline to the gel phase. A comparable downshift also occurs when DO3 is bound to cholesterol‐containing membranes in the liquid‐ordered phase. Our results demonstrate that Raman spectrum of DO3 is a unique tool for measuring the molecular order of lipids in membranes.
ISSN:2211-5463

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