Disperse Orange 3 as a resonance Raman probe for measuring membrane order
Resonance Raman spectra of azobenzene derivatives were examined in the presence of lipid membranes to find a probe that can distinguish different membrane phases. The NO2 symmetric stretching band of 4‐(4‐nitrophenylazo)aniline, also known as Disperse Orange 3 (DO3), is downshifted by about 4 cm−1 o...
| Published in: | FEBS Open Bio |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
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| Subjects: | |
| Online Access: | https://doi.org/10.1016/j.fob.2015.10.008 |
| _version_ | 1852682179928129536 |
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| author | Yuki Numakura Takashi Miura |
| author_facet | Yuki Numakura Takashi Miura |
| author_sort | Yuki Numakura |
| collection | DOAJ |
| container_title | FEBS Open Bio |
| description | Resonance Raman spectra of azobenzene derivatives were examined in the presence of lipid membranes to find a probe that can distinguish different membrane phases. The NO2 symmetric stretching band of 4‐(4‐nitrophenylazo)aniline, also known as Disperse Orange 3 (DO3), is downshifted by about 4 cm−1 on the phase transition of phosphatidylcholine membranes from the liquid crystalline to the gel phase. A comparable downshift also occurs when DO3 is bound to cholesterol‐containing membranes in the liquid‐ordered phase. Our results demonstrate that Raman spectrum of DO3 is a unique tool for measuring the molecular order of lipids in membranes. |
| format | Article |
| id | doaj-art-c2fcdfbb28f5400ca08f0bbf4ccabb2f |
| institution | Directory of Open Access Journals |
| issn | 2211-5463 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| spelling | doaj-art-c2fcdfbb28f5400ca08f0bbf4ccabb2f2025-08-19T21:28:07ZengWileyFEBS Open Bio2211-54632015-01-015185986310.1016/j.fob.2015.10.008Disperse Orange 3 as a resonance Raman probe for measuring membrane orderYuki Numakura0Takashi Miura1Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Sendai 980-8578, JapanGraduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Sendai 980-8578, JapanResonance Raman spectra of azobenzene derivatives were examined in the presence of lipid membranes to find a probe that can distinguish different membrane phases. The NO2 symmetric stretching band of 4‐(4‐nitrophenylazo)aniline, also known as Disperse Orange 3 (DO3), is downshifted by about 4 cm−1 on the phase transition of phosphatidylcholine membranes from the liquid crystalline to the gel phase. A comparable downshift also occurs when DO3 is bound to cholesterol‐containing membranes in the liquid‐ordered phase. Our results demonstrate that Raman spectrum of DO3 is a unique tool for measuring the molecular order of lipids in membranes.https://doi.org/10.1016/j.fob.2015.10.008Membrane orderDisperse Orange 3Raman spectroscopyResonance Raman probeMembrane microdomains |
| spellingShingle | Yuki Numakura Takashi Miura Disperse Orange 3 as a resonance Raman probe for measuring membrane order Membrane order Disperse Orange 3 Raman spectroscopy Resonance Raman probe Membrane microdomains |
| title | Disperse Orange 3 as a resonance Raman probe for measuring membrane order |
| title_full | Disperse Orange 3 as a resonance Raman probe for measuring membrane order |
| title_fullStr | Disperse Orange 3 as a resonance Raman probe for measuring membrane order |
| title_full_unstemmed | Disperse Orange 3 as a resonance Raman probe for measuring membrane order |
| title_short | Disperse Orange 3 as a resonance Raman probe for measuring membrane order |
| title_sort | disperse orange 3 as a resonance raman probe for measuring membrane order |
| topic | Membrane order Disperse Orange 3 Raman spectroscopy Resonance Raman probe Membrane microdomains |
| url | https://doi.org/10.1016/j.fob.2015.10.008 |
| work_keys_str_mv | AT yukinumakura disperseorange3asaresonanceramanprobeformeasuringmembraneorder AT takashimiura disperseorange3asaresonanceramanprobeformeasuringmembraneorder |