Guide for phenotype-specific profiling of DNA G-quadruplex-regulated genes
Summary: DNA G-quadruplex (G4) is a non-canonical four-stranded secondary structure that has been shown to play a role in epigenetic modulation of gene expression. Here, we present a primer on phenotype-specific profiling of DNA G-quadruplex-regulated genes. We provide guidance on in silico explorat...
| الحاوية / القاعدة: | STAR Protocols |
|---|---|
| المؤلفون الرئيسيون: | , , , |
| التنسيق: | مقال |
| اللغة: | الإنجليزية |
| منشور في: |
Elsevier
2024-03-01
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| الموضوعات: | |
| الوصول للمادة أونلاين: | http://www.sciencedirect.com/science/article/pii/S2666166723007876 |
| _version_ | 1851944558550581248 |
|---|---|
| author | Zhuoyang Zhao Jianru Wang Huichuan Yu Xiaolin Wang |
| author_facet | Zhuoyang Zhao Jianru Wang Huichuan Yu Xiaolin Wang |
| author_sort | Zhuoyang Zhao |
| collection | DOAJ |
| container_title | STAR Protocols |
| description | Summary: DNA G-quadruplex (G4) is a non-canonical four-stranded secondary structure that has been shown to play a role in epigenetic modulation of gene expression. Here, we present a primer on phenotype-specific profiling of DNA G-quadruplex-regulated genes. We provide guidance on in silico exploration of G4-related genes and phenotypes, and in vitro and in vivo validation of the relationship between G4 and phenotype. We describe commonly utilized techniques and detail critical steps involved in determining the phenotype-specific G4-regulated genes for subsequent investigations. |
| format | Article |
| id | doaj-art-c54520bc8d1647acb2ec0c767d6bd555 |
| institution | Directory of Open Access Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| spelling | doaj-art-c54520bc8d1647acb2ec0c767d6bd5552025-08-19T21:49:06ZengElsevierSTAR Protocols2666-16672024-03-015110282010.1016/j.xpro.2023.102820Guide for phenotype-specific profiling of DNA G-quadruplex-regulated genesZhuoyang Zhao0Jianru Wang1Huichuan Yu2Xiaolin Wang3Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Guangdong Institute of Gastroenterology, Guangzhou, Guangdong 510655, China; Department of Spine Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China; Guangdong Province Key Laboratory of Orthopedics and Traumatology, Guangzhou 510080, ChinaDepartment of Spine Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China; Guangdong Province Key Laboratory of Orthopedics and Traumatology, Guangzhou 510080, ChinaDepartment of General Surgery (Colorectal Surgery), The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Guangdong Institute of Gastroenterology, Guangzhou, Guangdong 510655, China; Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Key Laboratory of Human Microbiome and Chronic Diseases (Sun Yat-sen University), Ministry of Education, Guangzhou, Guangdong 510655, China; Corresponding authorDepartment of General Surgery (Colorectal Surgery), The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Guangdong Institute of Gastroenterology, Guangzhou, Guangdong 510655, China; Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China; Key Laboratory of Human Microbiome and Chronic Diseases (Sun Yat-sen University), Ministry of Education, Guangzhou, Guangdong 510655, China; Corresponding authorSummary: DNA G-quadruplex (G4) is a non-canonical four-stranded secondary structure that has been shown to play a role in epigenetic modulation of gene expression. Here, we present a primer on phenotype-specific profiling of DNA G-quadruplex-regulated genes. We provide guidance on in silico exploration of G4-related genes and phenotypes, and in vitro and in vivo validation of the relationship between G4 and phenotype. We describe commonly utilized techniques and detail critical steps involved in determining the phenotype-specific G4-regulated genes for subsequent investigations.http://www.sciencedirect.com/science/article/pii/S2666166723007876Sequence analysisGenomicsMolecular Biology |
| spellingShingle | Zhuoyang Zhao Jianru Wang Huichuan Yu Xiaolin Wang Guide for phenotype-specific profiling of DNA G-quadruplex-regulated genes Sequence analysis Genomics Molecular Biology |
| title | Guide for phenotype-specific profiling of DNA G-quadruplex-regulated genes |
| title_full | Guide for phenotype-specific profiling of DNA G-quadruplex-regulated genes |
| title_fullStr | Guide for phenotype-specific profiling of DNA G-quadruplex-regulated genes |
| title_full_unstemmed | Guide for phenotype-specific profiling of DNA G-quadruplex-regulated genes |
| title_short | Guide for phenotype-specific profiling of DNA G-quadruplex-regulated genes |
| title_sort | guide for phenotype specific profiling of dna g quadruplex regulated genes |
| topic | Sequence analysis Genomics Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723007876 |
| work_keys_str_mv | AT zhuoyangzhao guideforphenotypespecificprofilingofdnagquadruplexregulatedgenes AT jianruwang guideforphenotypespecificprofilingofdnagquadruplexregulatedgenes AT huichuanyu guideforphenotypespecificprofilingofdnagquadruplexregulatedgenes AT xiaolinwang guideforphenotypespecificprofilingofdnagquadruplexregulatedgenes |