δ-tocotrienol Inhibits Proliferation of Cervical Carcinoma Cells Through Upregulating MicroRNA-34a

• Published in: Zhongliu Fangzhi Yanjiu
• Main Authors: YU Ying, HUANG Jing, LI Rong, LIU Lianbin, WANG Qi, LUO Deping
• Format: Article
• Language: Chinese
• Published: Magazine House of Cancer Research on Prevention and Treatment 2018-12-01
• Subjects: cervical carcinoma; δ-tocotrienol; mir-34a; proliferation; ccnd1
• Online Access: http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0523.htm
• ISSN: 1000-8578; 1000-8578

Objective To determine the effects of δ-tocotrienol on the proliferation of cervical carcinoma HeLa cells and whether miR-34a is involved in this process. Methods The expression of miR-34a and proliferation-related genes, cell viability, the protein levels of proliferation-related genes and cell cycle were examined using Real-time PCR, CCK-8 assay, Western blot and flow cytometry, respectively. miR-34a mimics and inhibitors were used to determine whether miR-34a mediated the effects of δ-tocotrienol on cell proliferation. Results δ-tocotrienol treatment for 36h significantly decreased the viability of HeLa cells in a dose-dependent manner. δ-tocotrienol treatment at 20 μmol/L-1 for 36h significantly stimulated miR-34a expression, however, inhibited CCND1 expression; meanwhile, cells proportion in S phase was decreased. Besides, after miR-34a mimics transfection, the cell viability and CCND1 expression were also decreased. Furthermore, miR-34a inhibitor transfection attenuated the inhibitory effects of δ-tocotrienol on cell proliferation. Conclusion δ-tocotrienol inhibits the proliferation of cervical carcinoma cells through upregulating miR-34a expression.